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首页> 外文期刊>Avian Diseases >Isolation and Identification of Highly Pathogenic Avian Influenza Virus Subtype H5N1 in Peafowl (Pavo cristatus)
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Isolation and Identification of Highly Pathogenic Avian Influenza Virus Subtype H5N1 in Peafowl (Pavo cristatus)

机译：孔雀（Pavo cristatus）高致病性禽流感病毒H5N1亚型的分离与鉴定

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摘要

An outbreak of highly pathogenic avian influenza (HPAI) virus subtype H5N1 was first diagnosed in a "backyard" flock of peafowl (Polio cristatus) raised on palace premises in the Kingdom of Saudi Arabia in December 3, 2007. The flock consisted of 40 peafowl, and their ages ranged from 3 to 5 years old. Affected birds suffered from depression, anorexia, and white diarrhea. Four dead birds were submitted for HPAI diagnosis at the Central Veterinary Diagnostic Laboratory in Riyadh. Brain and liver tissues and tracheal and cloacal swabs were taken from the dead birds and processed for a real-time reverse transcriptase (RT)-PCR test and virus isolation in specific-pathogen-free embryonating chicken eggs. The H5N1 subtype of avian influenza virus was isolated from the four dead birds and identified by a real-time RT-PCR before and after egg inoculation. The virus isolates were characterized as HPAI H5N1 virus by sequencing analysis. Phylogenetic comparisons revealed that the H5N1 viruses isolated from peafowl belong to the genetic chide 2.2 according to the World Health Organization nomenclature. The peafowl H5N1 virus falls into 2.2.2 sublineage II and clusters with the H5N1 viruses isolated from poultry in Saudi Arabia in 2007-08.
机译：首次在2007年12月3日在沙特阿拉伯王国宫殿内饲养的“后院”孔雀（Polio cristatus）群中诊断出高致病性禽流感（HPAI）H5N1亚型暴发。该群包括40只孔雀。 ，他们的年龄介于3至5岁之间。患病的鸟类患有抑郁症，厌食症和白色腹泻。利雅得中央兽医诊断实验室将四只死鸟送交HPAI诊断。从死鸟中取出脑和肝组织以及气管和泄殖腔拭子，并进行实时逆转录酶（RT）-PCR测试，并在不含特定病原体的雏鸡卵中分离病毒。从四只死亡的禽类中分离出H5N1亚型禽流感病毒，并在接种鸡蛋前后通过实时RT-PCR进行鉴定。通过测序分析将病毒分离株表征为HPAI H5N1病毒。系统发育比较表明，根据世界卫生组织的命名法，从孔雀分离出的H5N1病毒属于2.2基因。孔雀H5N1病毒属于2.2.2亚类II，与2007-08年从沙特阿拉伯的家禽中分离出的H5N1病毒聚在一起。

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