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首页> 外文期刊>Avian Pathology >Quantification of parasite shedding and horizontal transmission parameters in Histomonas meleagridis-infected turkeys determined by real-time quantitative PCR
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Quantification of parasite shedding and horizontal transmission parameters in Histomonas meleagridis-infected turkeys determined by real-time quantitative PCR

机译：实时定量PCR确定的组织感染肉食性组织的火鸡中寄生虫脱落和水平传播参数的定量

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摘要

To gain more insight into the within flock transmission of Histomonas meleagridis, the shedding of parasites was quantified by a newly developed real-time quantitative (q)PCR and the basic reproduction number (R-0) and the mean number of secondary infections per infectious bird per day in a susceptible population () of H. meleagridis in the absence of heterakis were assessed. Forty turkeys were divided into two groups of 10 and 30 birds at 14 days of age. Birds of the first group were inoculated with 200,000 histomonads each, the second group served as a susceptible contact group. Cloacal swabs were taken at -1, 1, 4, 7, 9, 11, 14, 18 and 21 days post inoculation (p.i.) to assess the shedding of the parasite by the qPCR (detection limit 330 histomonads/ml droppings). The experiment ended at 28 days p.i. Mortality was 100% in the inoculated birds and started at day 12 p.i., while in the contacts, it was 83% and started at 16 days p.i. Shedding started 1 day after the inoculation in both groups. The mean shedding levels (and 95% CI) expressed as parasite equivalents per gram cloacal content on a log(10) scale in the inoculated, contact birds that died and contact birds alive were 2.0 (1.6-2.4), 1.6 (1.4-1.9) and 1.2 (0.5-2.0), respectively. Birds that died shed histomonas more often and were infectious for 13.4 days; in contrast, those that recovered were infectious for 5.7 days. R-0 was estimated to be 8.4 and 0.70. Simulations made with the parameters obtained were in agreement with the experimental results, confirming their validity.
机译：为了更深入地了解肉食性组织虫的群内传播，通过新开发的实时定量（q）PCR和基本繁殖数（R-0）以及每个传染病的继发感染平均数对寄生虫的脱落进行了定量在没有杂种动物的情况下，评估了易感性H. meleagridis种群中每天的家禽数量。 40只火鸡在14日龄时分为10和30只家禽两组。第一组的鸟类每只接种200,000个组织单胞菌，第二组作为易感接触组。在接种后的-1、1、4、7、9、11、14、18和21天（p.i.）采集泄殖腔拭子，以通过qPCR评估寄生虫的脱落（检出限为330组织细胞/毫升）。实验在下午28天结束。在接种的禽类中，死亡率为p.i. 12天开始的100％，而在接触者中，死亡率为83％，在p.i. 16天开始。两组接种后1天开始脱落。在接种，接触死亡和接触活禽的对数（10）等级上，以对数克重泄殖腔含量表示为每克泄殖腔含量的寄生虫当量的平均脱落水平（和95％CI）分别为2.0（1.6-2.4），1.6（1.4-1.9） ）和1.2（0.5-2.0）。死亡的鸟类更频繁地脱落组织瘤，并具有13.4天的传染性。相比之下，那些康复者感染了5.7天。 R-0估计为8.4和0.70。用获得的参数进行的仿真与实验结果一致，证实了它们的有效性。

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