• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Avian Diseases >Molecular detection and differentiation of infectious bursal disease virus
【24h】

Molecular detection and differentiation of infectious bursal disease virus

机译：传染性法氏囊病病毒的分子检测和分化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Vaccination of hens, with the subsequent maternal immunity imparted to chicks, is the primary means of controlling infectious bursal disease virus (IBDV). Effective vaccination depends on rapid and accurate diagnosis of the subtype present in a flock because vaccines based on the classic subtype of IBDV can fail to protect against challenge with a variant subtype. This review describes the various methods available to detect and differentiate between IBDV subtypes. Serotype 1 IBDV causes economically significant immunosuppressive disease in young chickens. Within serotype 1, two subtypes, classic and variant, can be differentiated by the virus neutralization assay. Antigen capture enzyme-linked immunosorbent assay (AC-ELISA) with MAbs has been successful at differentiating the very virulent IBDV phenotype (vvIBDV) from less pathogenic types. More rapid and sensitive molecular diagnostic methods based on reverse transcription-polymerase chain reaction (RT-PCR) for amplification of the IBDV VP2 gene have been a major focus of investigation in recent years. Conventional RT-PCR has been useful in detecting IBDV serotypes and, to a lesser extent, differentiating IBDV subtypes. One of the approaches has been the use of SspI and NgoM IV restriction enzymes, for restriction endonuclease (RE) analysis of RT-PCR products (RT-PCR-RE) and BstNI and MboI for restriction fragment length polymorphism (RFLP) analysis (RT-PCR-RFLP) to find unique banding patterns associated with antigenic variation within the variable region of the IBDV VP2 protein. However, these approaches were ultimately found to be unreliable because subtypes could not be consistently distinguished with restriction enzymes. These limitations led to studies in differentiating subtypes by detection of single nucleotide differences in sequence through real-time RT-PCR or DNA sequencing of RT-PCR products. Conventional RT-PCR, amplifying the VP2 hypervariable region, in combination with DNA sequencing of the PCR product, can differentiate classic, variant, and vvIBDV strains because variant and vvIBDV have characteristic nucleotide and amino acid substitutions. Real-time RT-PCR, targeting different regions of the IBDV genome, including VP1, VP2, and VP4 genes, in conjunction with melting-curve analysis is being investigated as a promising tool for molecular diagnosis of IBDV infection. These methods potentially allow for more rapid, sensitive, and specific detection and differentiation of IBDV classic, very virulent, and variant subtypes.
机译：母鸡的疫苗接种以及随后赋予雏鸡的母体免疫力，是控制传染性法氏囊病病毒（IBDV）的主要手段。有效的疫苗接种取决于对鸡群中亚型的快速，准确诊断，因为基于IBDV经典亚型的疫苗可能无法防止变异亚型的攻击。这篇综述描述了可用于检测和区分IBDV亚型的各种方法。血清型1 IBDV在雏鸡中引起经济上重要的免疫抑制疾病。在血清型1中，可以通过病毒中和测定来区分经典和变异两个亚型。单克隆抗体的抗原捕获酶联免疫吸附试验（AC-ELISA）已成功地将高毒力IBDV表型（vvIBDV）与致病性较低的类型区分开。近年来，基于逆转录聚合酶链反应（RT-PCR）的IBDV VP2基因扩增的更快速和灵敏的分子诊断方法已成为研究的重点。常规RT-PCR可用于检测IBDV血清型，并在较小程度上区分IBDV亚型。一种方法是使用SspI和NgoM IV限制性内切酶进行RT-PCR产物的限制性核酸内切酶（RE）分析（RT-PCR-RE），使用BstNI和MboI进行限制性片段长度多态性（RFLP）分析（RT） -PCR-RFLP），以找到与IBDV VP2蛋白可变区内的抗原变异相关的独特条带模式。然而，最终发现这些方法是不可靠的，因为不能用限制酶一致地区分亚型。这些局限性导致了通过实时RT-PCR或RT-PCR产物的DNA测序检测序列中单个核苷酸差异来区分亚型的研究。常规的RT-PCR扩增VP2高变区，结合PCR产物的DNA测序，可以区分经典，变异和vvIBDV菌株，因为变异和vvIBDV具有特征性的核苷酸和氨基酸取代。针对IBDV基因组的不同区域（包括VP1，VP2和VP4基因）的实时RT-PCR与熔解曲线分析一起被研究为IBDV感染分子诊断的有前途的工具。这些方法潜在地允许更快，更灵敏，更特异性地检测和区分IBDV经典，高毒力和变异亚型。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号