Sensitive and specific detection of influenza virus A subtype H5 with real-time PCR

Rossi J; Cramer S; Laue T

首页> 外文期刊>Avian Diseases >Sensitive and specific detection of influenza virus A subtype H5 with real-time PCR

【24h】

Sensitive and specific detection of influenza virus A subtype H5 with real-time PCR

机译：实时PCR灵敏，特异地检测H5亚型流感病毒

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Avian influenza is a serious threat to both animal and human health. To enable cutting-edge research in this field, we developed a molecular test on the basis of real-time polymerase chain reaction (real-time PCR), which detects influenza virus RNA. The test enables highly sensitive detection of influenza virus A and B strains, including H5N1, and specific identification of influenza A virus H5 subtypes. The kit was tested against a broad panel of influenza A and B subtypes and other respiratory viruses in collaboration with worldwide authoritative laboratories and shows a very high specificity and sensitivity. An internal control verifies RNA extraction as well as real-time PCR success. With this kit, rapid and reliable detection of influenza A and B viruses and identification of H5 subtypes can be achieved.

机译：禽流感是对动物和人类健康的严重威胁。为使该领域的前沿研究成为可能，我们在实时聚合酶链反应（实时PCR）的基础上开发了一种分子检测技术，该检测可检测流感病毒RNA。该测试能够高度敏感地检测包括H5N1在内的甲型和乙型流感病毒，并能特异性鉴定甲型H5流感亚型。该试剂盒与全球权威实验室合作，针对多种甲型和乙型流感亚型以及其他呼吸道病毒进行了测试，显示出非常高的特异性和敏感性。内部对照可验证RNA提取以及实时PCR成功。使用该试剂盒，可以快速可靠地检测甲型和乙型流感病毒并鉴定H5亚型。

著录项

来源
《Avian Diseases》 |2007年第1suppla期|共3页
作者
Rossi J; Cramer S; Laue T;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类动物医学（兽医学）;
关键词
Animals; Influenza A virus classification isolation amp; purification; Influenza B virus classification isolation amp; purification; Polymerase Chain Reaction methods; Reagent Kits; Diagnostic; Sensitivity and Specificity;

机译：动物;甲型流感病毒的分离和纯化;乙型流感病毒的分离和纯化;聚合酶链反应法;试剂盒;诊断;敏感性和特异性;

相似文献

外文文献
中文文献
专利

1. Sensitive and specific detection of influenza virus A subtype H5 with real-time PCR [J] . Rossi J, Cramer S, Laue T Avian Diseases . 2007,第1Suppla期

机译：实时PCR灵敏，特异地检测H5亚型流感病毒
2. Simultaneous detection of influenza virus type B and influenza A virus subtypes H1N1, H3N2, and H5N1 using multiplex real-time RT-PCR [J] . Fang Shisong, Li Jianxiong, Cheng Xiaowen, Applied Microbiology and Biotechnology . 2011,第4期

机译：使用多重实时RT-PCR同时检测B型流感病毒和A型流感病毒H1N1，H3N2和H5N1亚型
3. Multiplex one-step real-time PCR assay for rapid simultaneous detection of velogenic and mesogenic Newcastle disease virus and H5-subtype avian influenza virus [J] . Zhang Zhujun, Liu Dong, Hu Jiao, Archives of virology . 2019,第4期

机译：多路复用一步实时PCR测定，用于快速同时检测麝牛和脱蛋白疾病病毒和H5-亚型禽流感病毒
4. Comparison of Hemagglutinin and Neruaminidase of Influenza A Virus Subtype H1N1, H5N1, H5N2, and H7N9 Using Apriori Algorithm [C] . Dae Young Kim, Hye-Jun Kim, Junhyeok Bae, Pacific-Asia conference on knowledge discovery and data mining;International workshop on biologically inspired data mining techniques;International workshop on data analytics for targeted healthcare;International workshop on big data science and engineering on e-commerce;International workshop on mobile data management, mining and computing on social networks;International workshop on mobile sensing, mining and visualization for human behavior inferences;International workshop on cloud service discovery;International conference on algorithms for large-scale information processing in knowledge discovery;International workshop on scalable dats analytics: thoery and algorithms;nternational workshop on data mining in biomedical informatics and healthcare;International workshop on data mining in social networks;International workshop on pattern mining and application of big data . 2014

机译：使用Apriori算法比较H1N1，H5N1，H5N2和H7N9甲型流感病毒的血凝素和神经氨酸酶
5. Impedance Biosensors for the Rapid Detection of Viral and Bacterial Pathogens Using Avian Influenza Virus Subtypes H5N1 and H7N2 and Escherichia coli O157:H7 as Model Targetss. [D] . Lum, Jacob David. 2014

机译：使用禽流感病毒亚型H5N1和H7N2和大肠杆菌O157：H7作为模型目标的快速检测病毒和细菌病原体的阻抗生物传感器。
6. Development and Validation of a One-Step Real-Time PCR Assay for Simultaneous Detection of Subtype H5 H7 and H9 Avian Influenza Viruses [O] . Isabella Monne, Silvia Ormelli, Annalisa Salviato, 2008

机译：同时检测亚型H5H7和H9禽流感病毒的一步式实时PCR检测方法的开发和验证
7. Multiplex one-step Real-time PCR by Taqman-MGB method for rapid detection of pan and H5 subtype avian influenza viruses. [O] . Zhujun Zhang, Dong Liu, Wenqiang Sun, 2017

机译：通过Taqman-mGB方法进行多步一步实时pCR，快速检测泛和H5亚型禽流感病毒。

Sensitive and specific detection of influenza virus A subtype H5 with real-time PCR

摘要

著录项

相似文献

相关主题

期刊订阅