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首页> 外文期刊>Avian Pathology >Identification of chicken enterovirus-like viruses, duck hepatitis virus type 2 and duck hepatitis virus type 3 as astroviruses
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Identification of chicken enterovirus-like viruses, duck hepatitis virus type 2 and duck hepatitis virus type 3 as astroviruses

机译：鉴定鸡肠病毒样病毒，2型鸭肝炎病毒和3型鸭肝炎病毒为星状病毒

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Earlier work identified and biologically characterized antigenically distinct enterovirus-like viruses (ELVs) of chickens. Three of these ELVs can now be identified as astroviruses. Characterization involved the use of a hitherto undescribed, degenerate primer-based reverse transcription-polymerase chain reaction (RT-PCR) to amplify astrovirus open reading frame (ORF) 1b-specific cDNA fragments followed by nucleotide sequence determination and analysis of the amplified fragments. ELV-1 was confirmed as an isolate of the astrovirus avian nephritis virus (ANV). ELV-4 (isolate 612) and ELV-3 (isolates FP3 and 11672) were antigenically and genetically related to the second characterized astrovirus of chickens, namely chicken astrovirus (CAstV). Using indirect immunofluorescence, the FP3 and 11672 ELV-3 isolates were very closely related to one another, and less closely related to ELV-4 and the previously described CAstV (P22 18.8.00 reference isolate). Comparative analyses based on the ORF 1b amplicon sequences showed that the FP3 and 11672 ELV-3 isolates shared high nucleotide (95%) and amino acid (98%) identities with one another, and lower nucleotide (76% to 79%) and amino acid (84% to 85%) identity levels with ELV-4 and the reference CAstV P22 18.8.00 isolates. The combined degenerate primer RT-PCR and sequencing methods also provided a nucleotide sequence specific to duck hepatitis virus type 2 (DHV-2) (renamed duck astrovirus) and duck hepatitis virus type 3 (DHV-3), which, for the first time, can also be identified as an astrovirus. Phylogenetic analyses based on the amplified ORF 1b sequences showed that ANV was the most distantly related avian astrovirus, with DHV-3 being more closely related to turkey astrovirus type 2 than DHV-2.
机译：早期的工作鉴定了鸡的抗原性独特的肠病毒样病毒（ELV）并对其进行了生物学表征。这些ELV中的三个现在可以识别为星状病毒。表征涉及使用迄今未描述的，基于简并引物的逆转录聚合酶链反应（RT-PCR）扩增星状病毒开放阅读框（ORF）1b特异性cDNA片段，然后进行核苷酸序列测定和扩增片段的分析。 ELV-1被确认为星状病毒禽肾炎病毒（ANV）的分离物。 ELV-4（分离株612）和ELV-3（分离株FP3和11672）与鸡的第二种特征性星状病毒，即鸡星状病毒（CAstV）在抗原和遗传上相关。使用间接免疫荧光，FP3和11672 ELV-3分离株彼此非常紧密相关，而与ELV-4和先前描述的CAstV（P22 18.8.00参考分离株）之间的相关性较低。基于ORF 1b扩增子序列的比较分析表明，FP3和11672 ELV-3分离株彼此具有高核苷酸（95％）和氨基酸（98％）同一性，而较低核苷酸（76％至79％）和氨基酸（84％至85％）与ELV-4和参考CAstV P22 18.8.00分离株的同一性水平。结合的简并引物RT-PCR和测序方法还提供了特异于2型鸭肝炎病毒（DHV-2）（更名为鸭星状病毒）和3型鸭肝炎病毒（DHV-3）的核苷酸序列，这是首次，也可以识别为星状病毒。根据扩增的ORF 1b序列进行的系统进化分析表明，ANV是最密切相关的禽星状病毒，与DHV-2相比，DHV-3与2型火鸡星状病毒更紧密相关。

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