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首页> 外文期刊>Avian Pathology >Detection and localization of a goose adenovirus in experimentally infected goslings, using indirect immunofluorescence with paraffin-embedded tissue sections
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Detection and localization of a goose adenovirus in experimentally infected goslings, using indirect immunofluorescence with paraffin-embedded tissue sections

机译：使用间接免疫荧光法和石蜡包埋的组织切片，在实验感染的雏鹅中检测和定位鹅腺病毒

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摘要

To establish an ideal method for the detailed definition of the tropism of the goose adenovirus new-type gosling viral enteritis virus (NGVEV) in conventional paraformaldehyde-fixed paraffin-embedded gosling tissue sections, an indirect immunofluorescence assay was established and optimized in this study for the first time. Three-day-old goslings orally inoculated with the CN strain of NGVEV were killed from 0.5 h to 30 days post-infection (p.i.) at intervals, and organs were collected and prepared for immunofluorescence assay and ultrastructural observation. NGVEV antigens could be detected in the gastrointestinal tract and lymphoid organs as early as 24 h p.i. The lymphoid organs (bursa of Fabricius, thymus, Harderian gland and spleen), the digestive organs (proventriculus, gizzard, duodenum, jejunum, ileum, caecum, rectum and liver), kidney, myocardium and brain of NGVEV-infected goslings showed extensive evidence of presence of viral antigens. Lymphocytes, macrophages, mesenchymes, endothelial cells, epithelia, superficial and crypt mucosal cells, glandular cells, and fibrocytes served as the principal site for NGVEV localization. The number of positive cells and intensity of fluorescence increased until 15 days p.i. Ultrastructural observation showed that NGVEV particles could be seen from 3 days p.i. onwards.
机译：为了在常规多聚甲醛固定石蜡包埋的雏鹅组织切片中建立详细定义鹅腺病毒新型雏鹅病毒性肠炎病毒（NGVEV）趋向性的理想方法，本研究建立了间接免疫荧光测定法并对其进行了优化，以用于第一次。在感染后0.5小时至30天（p.i.）间隔杀死口服接种了NGVEV的CN菌株的三天大的幼鹅，收集器官并准备进行免疫荧光测定和超微结构观察。 NGVEV抗原最早可在p.i 24小时在胃肠道和淋巴器官中检测到。淋巴器官（法氏囊，胸腺，哈代腺和脾脏的法氏囊），消化器官（前肠，胃，十二指肠，空肠，回肠，盲肠，直肠和肝脏），肾脏，心肌和大脑受到NGVEV感染的雏鹅的证据表明病毒抗原的存在。淋巴细胞，巨噬细胞，间充质，内皮细胞，上皮，浅表和隐窝黏膜细胞，腺细胞和纤维细胞是NGVEV定位的主要部位。阳性细胞数量和荧光强度一直增加到p.i持续15天。超微结构观察表明，从下午3天开始可以看到NGVEV颗粒。向前。

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