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首页> 外文期刊>Biocontrol Science and Technology >Using particle size analysis to determine the hydrophobicity and suspension of fungal conidia with particular relevance to formulation of biopesticide
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Using particle size analysis to determine the hydrophobicity and suspension of fungal conidia with particular relevance to formulation of biopesticide

机译：使用粒度分析来确定真菌分生孢子的疏水性和悬浮性，与生物农药的配制特别相关

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Fungal formulations are vital for effective biopesticide development. Good formulations help to optimise field efficacy, while poor formulations result in product failure. This study aimed to produce a hydrophobicity test that would be appropriate for fungal conidia produced to a commercial quality and determine relative hydrophobicity of fungi from four different genera by using laser diffraction. A particle size analyser was used to determine the hydrophobicity of: three Metarhizium acridum samples, M. anisopliae, Beauveria bassiana, Trichoderma stromaticum, T. harzianum, T. viride and Alternaria eichhorniae conidia by suspending the conidia in three different liquids: Shellsol T (a mineral oil), water and 0.05 % Tween 80. Hydrophobicity was determined by the size of the particles formed in each of the liquids. All the Metarhizium samples were the most hydrophobic followed by B. bassiana and A. eichhorniae. The Trichoderma samples were the least hydrophobic. As a comparison, a phase exclusion assay and a salt-mediated aggregation and sedimentation (SAS) test were performed. It was not possible to get a reliable reading for the B. bassiana, A. eichhorniae and T. viride samples using the phase exclusion assay. The addition of salt in the SAS test did not affect the rate of sedimentation. It was hypothesised that conidia size affected the results of the SAS test that made A. eichhorniae the most hydrophobic conidia. Particle size analysis (PSA) was a more accurate test for comparing fungi from difference genera compared to the SAS test and phase exclusion assay. PSA was also used to test three emulsions and demonstrated that different formulations had an effect on particle size.
机译：真菌制剂对于有效的生物农药开发至关重要。好的配方有助于优化田间药效，而差的配方会导致产品失效。这项研究旨在进行疏水性测试，该测试适用于以商业质量生产的真菌分生孢子，并通过使用激光衍射确定来自四个不同属的真菌的相对疏水性。通过将分生孢子悬浮在三种不同的液体中，使用粒度分析仪确定以下分生孢子的疏水性：三种分生孢子菌，M。anisopliae，球孢白僵菌，间质木霉，间质衣原体T. harzianum，衣原体T.viride和交链孢菌。矿物油，水和0.05％的Tween80。疏水性取决于每种液体中形成的颗粒大小。所有的金属根样品都具有最大的疏水性，其次是球孢白僵菌和凤眼莲。木霉样品的疏水性最低。作为比较，进行了相排斥测定和盐介导的聚集和沉淀（SAS）测试。使用相排阻测定法无法获得球孢白僵菌，棘孢曲霉和拟南芥的可靠读数。在SAS测试中添加盐不会影响沉淀速率。假设分生孢子的大小会影响SAS试验的结果，该结果使凤尾曲霉成为最疏水的分生孢子。粒度分析（PSA）是比较差异属真菌的更准确测试，与SAS测试和相排除法相比。 PSA还用于测试三种乳液，并证明不同的配方会对粒径产生影响。

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