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首页> 外文期刊>Biocontrol Science and Technology >Utilisation of response surface methodology to optimise the culture medium for Bacillus thuringiensis subsp israelensis
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Utilisation of response surface methodology to optimise the culture medium for Bacillus thuringiensis subsp israelensis

机译：利用响应面法优化苏云金芽孢杆菌以色列亚种的培养基

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摘要

Bacillus thuringiensis israelensis (Bti) is a sporulating Gram-positive bacterium that produces protein crystals with insecticide activity against Diptera. The aim of the present work was to optimise the culture medium for this bacterium, based on mathematical and statistical concepts (factorial designs and response surface methodology). The variables studied were carbon and nitrogen source concentrations. The main response analysed was toxicity, evaluated by means of bioassay with Aedes aegypti. The nutrient sources were first selected and then optimised. Ground Bombyx mori pupae, ammonium sulphate and glucose were the most suitable sources of organic nitrogen, inorganic nitrogen and organic carbon, respectively. The toxicity of optimised medium (LC50 = 0.703 ppm,v/v) was higher than that the medium used as reference (LC50 = 3.01 ppm, v/v), which is commonly used in the laboratory culture of Bti. Besides, the optimised medium showed a cost 7.36 times less than that of an alternative medium, based on soybean flour and sugarcane molasses. Factorial design and response surface methodology were effective methods for culture medium optimisation. The results will contribute to the development of local production and utilisation of agroindustrial waste locally.
机译：苏云金芽孢杆菌以色列（Bti）是一种孢子形成革兰氏阳性细菌，可产生对双翅目具有杀虫活性的蛋白质晶体。本工作的目的是基于数学和统计概念（因子设计和响应面方法）来优化该细菌的培养基。研究的变量是碳和氮源浓度。分析的主要反应是毒性，通过埃及伊蚊的生物测定评估。首先选择营养来源，然后进行优化。地面家蚕p，硫酸铵和葡萄糖分别是最合适的有机氮，无机氮和有机碳来源。优化培养基（LC50 = 0.703 ppm，v / v）的毒性高于用作参考的培养基（LC50 = 3.01 ppm，v / v）的毒性，后者是Bti实验室培养中常用的。此外，以大豆粉和甘蔗糖蜜为基础，优化培养基的成本是替代培养基的7.36倍。析因设计和响应面方法是优化培养基的有效方法。结果将有助于当地生产的发展和当地农用废弃物的利用。

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