The enthalpy of helix-coil transition of DNA (#x394;H) is determined from the experiments on DNA melting with ligands by means of absolutely general formula, which contains only values directly known from the experiment (M.D. Frank-Kamenetskii, and A.T. Karapetian,Mol. Biol. USSR 6, 621 (1972)) with the combination of the #x201C;area#x201D; method (P.O. Vardevanian,et al, Biophysica 28, 130 (1983)). The experimentally obtained data show that #x394;H depends on both concentration of Na+in solution and GC-content of DNA and is of high accuracy.
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