...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Indian Journal of Ecology >Efficient In vitro Sterilization Technique for Micropropagation of Banana (Musa acuminata) cv. 'Grand Name'
【24h】

Efficient In vitro Sterilization Technique for Micropropagation of Banana (Musa acuminata) cv. 'Grand Name'

机译：香蕉（Musa acuminata）cv微繁殖的高效体外灭菌技术。 “全名”

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Banana (Musa spp.) is the fourth most important food commodity that grows throughout in humid tropics and subtropics with an annual production of 97.5 million tonnes (Ganapathi et al., 2008). Application of micropropagation technique for large scale production of elite clones of banana is an effective and superior alternative to propagation through conventional cuttings of Musa spp. In vitro propagation technique for banana (Musa acuminata L.) cv. 'Grand Naine' involves various steps, i.e., establishment of aseptic cultures, shoot multiplication, induction of rooting, hardening and transfer of plantlets to soil. The maintenance of aseptic (free from all microorganisms) or sterile conditions is essential for successful tissue culture procedures. To maintain an aseptic environment, all culture vessels, media and instruments used in handling tissues, as well as explant itself must be sterilized. Various sterilization agents are used to decontaminate the tissues. These sterilants are also toxic to theplant tissues, hence proper concentration of sterilants, duration of exposing the explant to the various sterilants, the sequences of using these sterilants has to be standardized to minimize explant injury and achieve better survival. Two different chemicals, 0.1% carbendazim (Bavistin from BASF India Ltd, Mumbai) and mercuric chloride (HgCI2) were used for the present study to reduce the incidence of both fungal and bacterial contamination and to standardize the best sterilization protocol for in vitro culture of banana cv. 'Grand Naine'.
机译：香蕉（Musa spp。）是在热带和亚热带湿润地区第四大最重要的食品，年产量达9750万吨（Ganapathi等，2008）。微繁殖技术在香蕉优良无性系大规模生产中的应用，是通过常规芭蕉属插条繁殖的有效且优越的替代方法。香蕉（Musa acuminata L.）cv。的体外繁殖技术。 'Grand Naine'涉及多个步骤，即建立无菌培养物，枝条繁殖，诱导生根，硬化以及将小植株转移到土壤中。维持无菌（不含所有微生物）或无菌条件对于成功的组织培养程序至关重要。为了保持无菌环境，必须对所有用于处理组织的培养容器，培养基和仪器以及外植体本身进行灭菌。各种消毒剂用于净化组织。这些灭菌剂也对植物组织有毒，因此灭菌剂的浓度适当，将外植体暴露于各种灭菌剂的持续时间，必须标准化使用这些灭菌剂的顺序，以最大程度地减少外植体的伤害并获得更好的存活率。本研究使用0.1％多菌灵（孟买的BASF印度公司的Bavistin）和氯化汞（HgCl2）两种不同的化学物质，以减少真菌和细菌污染的发生率，并标准化用于体外培养的最佳灭菌方案。香蕉简历'大纳​​奈'。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号