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首页> 外文期刊>Indian Journal of Agricultural Biochemistry >Assessment of Genetic Diversity among Chickpea Genotypes using RAPD, Protein Profiling and Isozyme Markers
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Assessment of Genetic Diversity among Chickpea Genotypes using RAPD, Protein Profiling and Isozyme Markers

机译：利用RAPD，蛋白质谱和同工酶标记评估鹰嘴豆基因型之间的遗传多样性

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摘要

The genetic diversity and similarity were investigated in 24 chickpea {Cicer arietinum L.) genotypes using dominant markers viz. RAPD, protein profiling and isozymes markers. Total 118 amplicons were produced by fourteen RAPD marker, of which 90 (76.27%) were found polymorphic. Leaf protein profiling of genotypes had produced 9 (45%) polymorphic bands across the set. Three different isozymes viz. esterase, peroxidase and acid phosphatase were studied, of which only the esterase isozyme was found polymorphic (55.55 %). Total 154 scorabie bands were generated using 18 different markers, out of which 112 bands were observed to be polymorphic (72.73 %). Highest polymorphism was seen with RAPD primers, while it was least with the isozymes. Based on the jaccard's similarity coefficient, the similarity index was observed in the range of 0.6222 to 0.9427 among 24 chickpea genotypes. The lowest genetic similarity was observed between BGT-300 and ACP 417. The dendrogram of chickpea genotypes showed two majorclusters. The genotypes Godra nazir, Bavsar and.BGT 300 were grouped in one cluster. It was found that the Desiand Kabulitypes did not segregate into two distinct groups.
机译：遗传多样性和相似性调查了24种鹰嘴豆（Cicer arietinum L.）基因型，使用显性标记。 RAPD，蛋白质谱和同工酶标记。 14个RAPD标记共产生118个扩增子，其中90个（76.27％）被发现是多态的。基因型的叶蛋白谱分析在整个植物中产生了9个（45％）多态性条带。三种不同的同工酶。对酯酶，过氧化物酶和酸性磷酸酶进行了研究，其中只有酯酶同工酶是多态的（55.55％）。使用18个不同的标记产生了154条可伸缩带，其中112条带是多态性的（72.73％）。 RAPD引物观察到最高的多态性，同工酶观察到的最低。根据雅卡德的相似系数，在24种鹰嘴豆基因型中，相似指数在0.6222至0.9427范围内。在BGT-300和ACP 417之间观察到最低的遗传相似性。鹰嘴豆基因型的树状图显示出两个主要簇。基因型Godra nazir，Bavsar和.BGT 300归为一类。发现Desiand Kabulitypes并没有分成两个不同的组。

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