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首页> 外文期刊>Indian Journal of Agricultural Biochemistry >Varietal Identification of Sorghum (Sorghum bicolor (L.) Monech) Cultivars by RAPD Markers
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Varietal Identification of Sorghum (Sorghum bicolor (L.) Monech) Cultivars by RAPD Markers

机译：利用RAPD标记对高粱（Sorghum bicolor（L.）Monech）品种进行品种鉴定

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摘要

Biochemical and molecular techniques, isozyme pattern and random amplified polymorphic DNA (RAPD) analysis were applied in sorghum cultivars analysis to compare suitability for quantifying genetic diversity. Considering the limits of the use of morphological markers, the reliable estimate of DNA fingerprinting of the sorghum cultivars was assessed using isozyme banding pattern and RAPD analysis using random operon primers. Random amplified polymorphic DNA (RAPD) technique was used for genetic analysis of nine sorghum cultivars with different eight random operon primers. All eight operon primers were polymorphic and 67 amplified bands were obtained, out of which 44 were polymorphic, accounting 65.67% polymorphism. OPC 19 was most informative primer with 10 polymorphic bands, which can be used for further study of sorghum germplasm. Thus the results obtained on the polymorphism with different operon primers in relation to cultivars and the known pedigree information indicate the usefulness of the RAPD marker in determining genetic distance in sorghum. Peroxidase banding pattern was very prominent at Rf values 0.36, 0.50, 0.76 and 0.80. Banding patterns of peroxidase isozymes for M 35-1 and Selection - 3 was very dark than other genotypes. This typeof information can be helpful in basic identification of molecular differences in the various types of sorghum genotypes.
机译：将生化和分子技术，同工酶模式和随机扩增多态性DNA（RAPD）分析应用于高粱品种分析中，以比较适用于定量遗传多样性的情况。考虑到形态标记物使用的局限性，使用同工酶谱带模式和使用随机操纵子引物进行的RAPD分析评估了高粱品种DNA指纹的可靠估计。使用随机扩增多态性DNA（RAPD）技术对具有八个随机操纵子引物的九个高粱品种进行了遗传分析。全部8个操纵子引物均为多态性，获得67条扩增带，其中44条为多态性，占65.67％。 OPC 19是信息最丰富的引物，具有10个多态性条带，可用于高粱种质的进一步研究。因此，关于不同品种，使用不同操纵子引物进行多态性分析的结果以及已知的谱系信息表明，RAPD标记可用于确定高粱的遗传距离。 Rf值分别为0.36、0.50、0.76和0.80时，过氧化物酶带状谱非常突出。 M 35-1和Selection-3的过氧化物酶同工酶的带状谱比其他基因型暗。这种类型的信息可能有助于基本鉴定各种类型的高粱基因型中的分子差异。

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