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首页> 外文期刊>Indian Journal of Agricultural Biochemistry >DNA fingerprinting of pigeon pea (Cajanus cajan (L.) Millsp.) genotypes by RAPD marker for the breeding of new varieties.
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DNA fingerprinting of pigeon pea (Cajanus cajan (L.) Millsp.) genotypes by RAPD marker for the breeding of new varieties.

机译：利用RAPD标记对木豆（Cajanus cajan（L.）Millsp。）基因型进行DNA指纹识别，以育种新品种。

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摘要

Seven pigeon pea (Cajanus cajan) genotypes namely NDA-198-1, NDA-1, ICP-3424, IPA-2003-13, Bold Ram Krishna, ICP-3378 and ICP-2130 were selected for diversity assessment by RAPD marker. A total of 47 amplicons which were range from 300bp to 2510bp and an average of eight amplicons were amplified per primer. Highest similarity (81%) was found between NDA-1-98-1 and ICP-3378, followed by NDA-1 and IPA-2003-13 (80%), whereas minimum similarity (73%) was found in between Bold Ram Krishna and ICP-3378. The first major group was clustered with three pigeon pea genotypes NDA-1, ICP-3378 and Bold Ram Krishna. NDA-1-98-1 separated within same ICP-3378 separated clad in major group A. Bold Ram Krishna was found to be most diverse in all the assessed pigeon pea genotypes. NDA-1 has a specific band of 1550 bp which will be used for the development of SCAR marker for cultivar identification.
机译：通过RAPD标记选择7种木豆（Cajanus cajan）基因型NDA-198-1，NDA-1，ICP-3424，IPA-2003-13，Bold Ram Krishna，ICP-3378和ICP-2130进行多样性评估。每个引物总共扩增了47个扩增子，范围从300bp到2510bp，平均扩增了8个扩增子。发现NDA-1-98-1与ICP-3378之间的相似度最高（81％），其次是NDA-1与IPA-2003-13的相似度（80％），而在Bold Ram之间发现的最小相似度（73％）克里希纳和ICP-3378。第一大类群聚集了三种木豆基因型NDA-1，ICP-3378和Bold Ram Krishna。在主要A组中，在同一ICP-3378中分离的NDA-1-98-1分离，在所有评估的木豆基因型中，Bold Ram Krishna的差异最大。 NDA-1具有1550 bp的特异性条带，可用于开发SCAR标记以进行品种鉴定。

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