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Phytochemical Analysis of Malaxis acuminta D.Don (Jeevak), an Ingredient of Jeevaniya Group of Ashtavarga

机译:Ashtavarga的Jeevaniya集团的成分Malaxis acuminta D.Don(Jeevak)的植物化学分析

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The primary goal of present study was qualitative analysis and mass detection of Malaxis acuminta D.Don (Jeevak) with antiageing ethno-botanical records in northern India. Dry root samples of Malaxis acuminta D.Don were extracted with 9:1 methanol/water (MeOH/H20) solution. Sepbox 2D-2000, a multicolumn HPLC was used to separate the root extract with C 18 analytical column and mobile phase consisting of acetonitrile(ACN) MeOH H20 for each gradient resulted in 143 fractions. The method provides rapidand reproducible results. Screening was carried out by ELSD & UV detector data. After screening, 4 fractions were selected for gas chromatography/mass spectrometry (GC MS) analysis. Total 67 compounds with different retention time (RT), molecular weight(MW) and with different pharmaceutical activity were identified through GC MS analysis.
机译:本研究的主要目的是对印度北部具有抗衰老的植物学记录的Malaxis acuminta D.Don(Jeevak)进行定性分析和质量检测。用9:1甲醇/水(MeOH / H2O)溶液提取Malaxis acuminta D.Don的干燥根样品。使用多柱HPLC Sepbox 2D-2000,用C 18分析柱分离根提取物,每个梯度由乙腈(ACN)MeOH H2O组成的流动相分离得到143个馏分。该方法提供了快速且可重现的结果。通过ELSD和UV检测器数据进行筛选。筛选后,选择4个馏分进行气相色谱/质谱(GC MS)分析。通过GC MS分析鉴定了总共67种具有不同保留时间(RT),分子量(MW)和不同药物活性的化合物。

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