• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Indian Journal of Agricultural Biochemistry >The effect of fluoride on nitrate reductase activity in leaves of light and dark grown seedlings of wheat
【24h】

The effect of fluoride on nitrate reductase activity in leaves of light and dark grown seedlings of wheat

机译：氟对小麦光明和黑暗生长幼苗叶片硝酸盐还原酶活性的影响

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present study indicated that the addition of fluoride did not inhibit In vivo nitrate reductase activity in leaves of dark grown seedlings, while there was tremendous inhibition in leaves of light grown seedlings. Fluoride had no significant effect on in vitro nitrate reductase activity. Inhibition In leaves of light grown seedling in in vivo nitrate reductase activity increased with the enhanced concentration of fluoride in assay medium. The inhibition in in vivo nitrate reductase activity exerted by the fluoride in leaves of light grown seedlings was partially reversed by the addition of pyruvate, succinate and malate in assay mixture. The result indicated that NADH generated in the reaction occurring beyond the triosephosphate dehydrogenase step of glycolysis may be utilized in nitrate reduction in light. Experiment with leaves of dark grown seedlings showed that addition of glycolytic intermediates in assay medium did not inhibit the nitrate reductase enzyme activity. This suggested that the source of NADH in dark grown seedlings is from glycolytic pathway. In conclusion, the observed effect of fluoride on leaves of light and dark grown seedlings of wheat depended on the physiological availability of NADH.
机译：本研究表明，添加氟化物不会抑制深色幼苗的叶片体内硝酸盐还原酶活性，而对浅色幼苗的叶片具有极大的抑制作用。氟化物对体外硝酸还原酶活性没有明显影响。体内轻质幼苗叶片中的抑制作用硝酸还原酶活性随测定培养基中氟化物浓度的增加而增加。通过在分析混合物中添加丙酮酸，琥珀酸和苹果酸，部分逆转了氟化物在轻度生长幼苗的叶片中对体内硝酸盐还原酶活性的抑制作用。结果表明，在糖酵解的三糖磷酸脱氢酶步骤之后发生的反应中产生的NADH可用于光的硝酸盐还原。用深色幼苗的叶子进行的实验表明，在测定培养基中添加糖酵解中间体不会抑制硝酸还原酶的活性。这表明黑暗生长的幼苗中NADH的来源来自糖酵解途径。总之，观察到的氟化物对小麦的光明和黑暗生长幼苗叶片的影响取决于NADH的生理有效性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号