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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Regeneration of plant by somatic embryogenesis in Pinus rigida × P. taeda
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Regeneration of plant by somatic embryogenesis in Pinus rigida × P. taeda

机译：体细胞胚发生的硬松×taeda植株再生

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摘要

Zygotic embryos at different developmental stages were tested for their potential in the initiation of embryogenic suspensor mass (ESM) lines using immature seeds of Pinus rigida×P. taeda. The highest frequency (1.1%) of ESM was obtained with explants from cones collected on July 1. All excised embryos of the July 1 collection were at the early proembryo stage. Two different culture media were compared. Forty-eight ESM lines were initiated on Pinus taeda basal medium (P6) (0.97%) with 13.5 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.4 μM benzyladenine (BA). However, only four ESM were obtained on a modified Murashige and Skoog medium (MSG; 0.55%). Most of the ESM arose from the seeds that were at the stages ranging from late cleavage polyembryony to the early staged proembryo. Out of 52 lines (0.46%) that were produced from 11,388 explants, only two viable lines (0.018%) (PRT11 and PRT28) survived. As for somatic embryo maturation, the highest number (224/g?1 FW) of matured cotyledonary somatic embryos (line PRT 28) was obtained on a medium containing 100 μM abscisic acid (ABA), 0.2 M maltose, and 1.2% gellan gum. For germination of the somatic embryos, the cotyledonary somatic embryos derived from maturation medium were transferred on half-strength Litvay medium (LM) plus 0.4% gellan gum. The germination rates were high (71.4–96.3%) regardless of the concentrations of either ABA or gellan gum in the maturation medium. Approximately 500 somatic plants were recovered from the germination medium and transferred to the green house; finally most of them were transplanted successfully to the experimental field.
机译：测试了合子胚在不同发育阶段的潜力，使用未成熟松树种子×松树种子启动了胚性悬浮体（ESM）系。 taeda。 ESM的最高频率（1.1％）是从7月1日收集的视锥细胞中的外植体获得的。7月1日收集的所有切除的胚胎均处于早期胚胎早期。比较了两种不同的媒体。在具有13.5μM2,4-二氯苯氧基乙酸（2,4-D）和4.4μM苄腺嘌呤（BA）的针状松基础培养基（P6）（0.97％）上启动48条ESM系。但是，在改良的Murashige和Skoog培养基（MSG; 0.55％）上仅获得了四种ESM。 ESM的大部分来自种子，该种子处于从卵裂后期多胚到早期原胚的各个阶段。从11388个外植体产生的52个品系（0.46％）中，只有2个（0.018％）（PRT11和PRT28）可行品系存活。至于体细胞胚的成熟，在含有100μM脱落酸（ABA），0.2 M麦芽糖和1.2％吉兰糖胶的培养基上可获得最高数量（224 / g？1 FW）的成熟子叶体细胞胚（品系PRT 28）。 。为了使体细胞胚萌发，将来自成熟培养基的子叶体细胞胚转移到加有0.4％结冷胶的半强度Litvay培养基（LM）上。不论成熟培养基中ABA或结冷胶的浓度如何，发芽率都很高（71.4–96.3％）。从发芽培养基中回收了大约500种体细胞植物，并将其转移到温室中。最终他们中的大多数成功地移植到了实验领域。

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