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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Optimization of Renealmia mexicana (Klotzsch ex. Petersen) cultivation in vitro
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Optimization of Renealmia mexicana (Klotzsch ex. Petersen) cultivation in vitro

机译：墨西哥雷尼菌（Kertzsch ex.Petersen）体外培养的优化

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摘要

Renealmia mexicana (Klotzsch ex. Petersen) is a tropical plant found in southern México with an ornamental value and a potential source of curcuminoids. Its distribution in Chiapas has decreased because of deforestation and low propagation and germination rate, so a protocol for in vitro propagation was developed. An orthogonal experimental design of L9 (34) in triplicate was used to investigate the effect of 6-benzyl adenine (BA), indole butyric acid (IBA), silver nitrate (AgNO3), and sucrose on shoot, root, and leaf development of plantlets grown in vitro. Plantlets with well-developed shoots and roots were transferred to pots containing a mixture of peat moss and agrolite for hardening before transfer to soil. The Murashige and Skoog (Physiol. Plant. 15:473–497, 1962) mineral medium (MS) supplemented with 4.4 μM BA, 2.5 μM IBA, 11.7 μM AgNO3y and 5.5% (w/v) sucrose gave most shoots, 8.9 μM BA, 2.5 μM IBA, 17.7 μM AgNO3 and 5.5% (w/v) sucrose most roots, and 8.9 μM BA, 4.9 μM IBA, 11.7 μM AgNO3 and 3.0% (w/v) sucrose most leaves, although other combinations were statistically equivalent in each case. Sucrose was the factor that most explained the variation in the promotion of shoots, roots, and leaves. The protocol developed resulted in up to 100% survival when plantlets were transferred to soil using AgNO3, confirming that hardening of plantlets in vitro using hormonal stimulation was a suitable strategy to improve acclimatization.
机译：墨西哥雷尼氏菌（Renealmia mexicana）（Klotzsch ex。Petersen）是一种在墨西哥南部发现的热带植物，具有观赏价值和潜在的姜黄素来源。由于森林砍伐，繁殖和发芽率低，其在恰帕斯州的分布有所减少，因此制定了体外繁殖方案。一式三份的L9（34）的正交实验设计用于研究6-苄基腺嘌呤（BA），吲哚丁酸（IBA），硝酸银（AgNO3）和蔗糖对番茄茎，根和叶发育的影响体外生长的小植株。将具有成熟芽和根的小植株转移到装有泥炭藓和农用石土混合物的盆中进行硬化，然后再转移到土壤中。 Murashige and Skoog（Physiol。Plant。15：473–497，1962）矿物培养基（MS）补充有4.4μMBA，2.5μMIBA，11.7μMAgNO3y和5.5％（w / v）蔗糖可提供多数芽，8.9μM BA，2.5μMIBA，17.7μMAgNO3和多数蔗糖为5.5％（w / v）蔗糖，以及8.9μMBA，4.9μMIBA，11.7μMAgNO3和多数蔗糖为3.0％（w / v）蔗糖的多数根，尽管其他组合在统计学上在每种情况下都是等效的。蔗糖是最能解释芽，根和叶促进变化的因素。当使用AgNO3将小植株转移到土壤中时，制定的方案可导致高达100％的存活率，这证明使用激素刺激在体外使小植株变硬是改善适应性的合适策略。

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