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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >In vitro micropropagation of three rare, endangered, and endemic rhododendron species of Northeast India
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In vitro micropropagation of three rare, endangered, and endemic rhododendron species of Northeast India

机译：印度东北三种稀有，濒危和特有杜鹃物种的体外微繁殖

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摘要

In vitro propagation of three rare, endangered and endemic rhododendron species-Rhododendron dalhousiae var. rhabdotum, R. elliottii, and R. johnstoneanum-was attained. Nodal explants were used for multiple shoot induction studies. Three cytokinins (isopentenyladenine, benzyladenine, and kinetin) were evaluated in all three species. Isopentenyladenine performed better in all three species, especially at the concentration of 39.36 A mu M. Testing of combinations of growth regulators revealed that explants grown on Anderson medium supplemented with 39.36 A mu M isopentenyladenine and 4.90 A mu M indole butyric acid gave optimum results with 100% multiple shoot induction, 18 shoots per explant, and shoot length of 2.07 cm with R. johnstoneanum. Indole butyric acid was found to be the best auxin for root formation. Around 60% of the in vitro-raised plants of R. elliottii, R. johnstoneanum, and R. dalhousiae var. rhabdotum were able to establish ex vitro.
机译：三种稀有，濒危和特有的杜鹃花物种杜鹃花的体外繁殖。达到了大黄菊，elliottii和R. johnstoneanum。节点外植体用于多次芽诱导研究。在所有三个物种中评估了三种细胞分裂素（异戊烯腺嘌呤，苄基腺嘌呤和激动素）。异戊烯基腺嘌呤在所有三个物种中的表现都更好，尤其是在39.36 AμM的浓度下。对生长调节剂的组合进行的测试显示，在补充了39.36 AμM异戊烯基腺嘌呤和4.90 A M吲哚丁酸的Anderson培养基上生长的外植体在百分百诱导多枝芽，每株外植芽18枝，约翰内斯杜松（R. johnstoneanum）的芽长2.07 cm。发现吲哚丁酸是形成根的最佳生长素。 R. elliottii，R。johnstoneanum和R. dalhousiae var的体外培养植物中约有60％。横纹肌能够离体建立。

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