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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Effects of ionizing radiation on the growth and allyl isothiocyanate accumulation of Wasabia japonica in vitro and ex vitro
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Effects of ionizing radiation on the growth and allyl isothiocyanate accumulation of Wasabia japonica in vitro and ex vitro

机译：电离辐射对山葵生长和异硫氰酸烯丙酯积累的影响

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Mutations were induced in tissue-cultured wasabi (Wasabia japonica Matsumura) by treating in vitro-derived shoot tips with either γ-rays or X-rays at 0, 10, 20, 40 or 80 Gy. Doses of up to 40 Gy of either γ- or X-ray treatments resulted in a survival rate of more than 60% in culture after 3 mo. The use of γ- or X-rays at doses between 10 Gy and 40 Gy to induce mutation in W. japonica resulted in an alteration of the growth and allyl isothiocyanate (AITC) content of multiple shoots after 3 mo. in culture on Murashige and Skoog medium containing 5 μM N6-benzyladenine (BA). Putative mutants from the 40 Gy treatments of either γ- or X-rays exhibited a reduction in shoot weight, number, and height, whereas treatments of either γ-rays or X-rays at 10 Gy and 20 Gy doses showed no significant differences in shoot growth. All shoots treated with 80 Gy were either necrotic or irregenerable, while those treated with 40 Gy produced deformed leaves, from both types of ionizing radiation. Concentrations of AITC were measured by the use of gas chromatography-mass spectrometry (GC-MS). The accumulation of AITC was shown to decrease when doses increased in both γ- and X-ray treatments, compared with the controls. Positive responses were solely occurred at 18 mo. after transfer of in vitro rooted shoots to the shade house. The survival rate, rhizome weight and AITC content of plants derived from shoots treated with 20 Gy or 40 Gy of either γ-rays or X-rays were significantly greater than those of the controls.
机译：通过用0、10、20、40或80 Gy的γ射线或X射线处理体外衍生的芽尖，可以在组织培养的芥末（Wasabia japonica Matsumura）中诱导突变。剂量达40 Gy的γ射线或X射线治疗，在3 mo后的培养中存活率超过60％。在10 Gy和40 Gy之间的剂量下使用γ射线或X射线诱导粳稻的突变，导致3个月后多次芽的生长和异硫氰酸烯丙酯（AITC）含量发生变化。在含有5μMN6-苄基腺嘌呤（BA）的Murashige和Skoog培养基上培养。来自40 Gy处理的γ射线或X射线的推定突变体显示出芽重，数量和高度降低，而以10 Gy和20 Gy剂量处理的γ射线或X射线显示无显着差异。芽生长。用80 Gy处理的所有芽均是坏死的或不可再生的，而用40 Gy处理的芽则由于两种类型的电离辐射而产生变形的叶片。通过使用气相色谱-质谱法（GC-MS）测量AITC的浓度。与对照相比，当剂量在γ射线和X射线治疗中均增加时，显示AITC的累积减少。阳性反应仅在18 mo发生。将生根的芽转移到遮阴处后。用20 Gy或40 Gy的γ射线或X射线处理过的芽的植物的成活率，根茎重和AITC含量均显着高于对照。

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