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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Micropropagation and seed cryopreservation of the critically endangered species Tennessee yellow-eye grass, Xyris tennesseensis Kral.
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Micropropagation and seed cryopreservation of the critically endangered species Tennessee yellow-eye grass, Xyris tennesseensis Kral.

机译：极度濒危物种田纳西州黄眼草 tenriseensis Kral的微繁殖和种子低温保存。

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摘要

Xyris tennesseensis is a critically endangered species native to the southeastern USA. A micropropagation protocol was developed which may assist in the safeguarding and augmentation of dwindling natural populations of this ecologically and medically valuable plant. Four different batches of seeds were sterilized using hydrogen peroxide and germinated in vitro on modified one third-strength Murashige and Skoog medium. Shoot multiplication from seedling tissue was obtained using modified one third-strength Murashige and Skoog medium containing 1 mg/l kinetin and 0.1-0.5 mg/l alpha -naphthaleneacetic acid. Optimal shoot size and sustainable multiplication rates of three to five per 2-mo subculture occurred on medium containing 0.3-0.4 mg/l alpha -naphthaleneacetic acid. Shoots rooted successfully when placed on growth regulator-free medium for 10 d followed by transfer to greenhouse soil under high humidity. Use of seed cryopreservation resulted in significant increases in germination compared to control treatments with average germination rates of 97%. Shoot tip cultures from soil-grown plants of X. tennesseensis and Xyris spathifolia were also developed using the above protocols. Plant tissue culture tools will assist in the multiplication, long-term storage, and conservation of these rare and valuable plants as well as provide a template for the micropropagation of other Xyris species.
机译：tenriseensis（iy tennesseensis）是美国东南部的一种极度濒危物种。开发了微繁殖方案，其可以帮助保护和增加这种具有生态和医学价值的植物的自然种群的减少。使用过氧化氢对四批不同的种子进行灭菌，并在改良的一种第三强度的Murashige和Skoog培养基上在体外发芽。使用含有1 mg / l激动素和0.1-0.5 mg / lα-萘乙酸的改良的一种第三强度Murashige和Skoog培养基，可以从幼苗组织中获得芽繁殖。在含有0.3-0.4 mg / lα-萘乙酸的培养基上，每2个月亚培养具有3至5个最佳的芽长和可持续繁殖率。将芽置于无生长调节剂的培养基上10 d后成功生根，然后转移至高湿度的温室土壤中。种子冷冻保存的使用与平均发芽率为97％的对照处理相比，导致发芽量显着增加。从 X的土壤植物中拍摄尖端培养物。 Tennesseensis 和 Syris spathifolia 也是使用上述协议开发的。植物组织培养工具将有助于这些稀有和珍贵植物的繁殖，长期保存和保存，并为其他 Xyris 物种的微繁殖提供模板。

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