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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Over-expression of l-gulono-gamma-lactone oxidase (GLOase) gene leads to ascorbate accumulation with enhanced abiotic stress tolerance in tomato
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Over-expression of l-gulono-gamma-lactone oxidase (GLOase) gene leads to ascorbate accumulation with enhanced abiotic stress tolerance in tomato

机译：l-gulono-γ-内酯氧化酶（GLOase）基因的过表达导致抗坏血酸积累，增强了番茄的非生物胁迫耐受性

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Vitamin C (ascorbic acid, AsA) is an essential component for collagen biosynthesis and also for correct functioning of the cardiovascular system in humans. Plants and several animals can synthesize ascorbic acid, whereas humans lack the gene essential for ascorbic acid biosynthesis and must acquire the vitamin from their diet. In the present study, we developed a transgenic tomato plant (Lycopersicon esculentum Mill.) over-expressing the rat l-gulono-γ-lactone oxidase (GLOase) gene driven by CaMV35S constitutive promoter. The over-expression of GLOase resulted in a 1.5-fold increase in AsA levels in transgenic tomato fruits, which was positively correlated with increased GLOase activity. The Southern and reverse transcriptase polymerase chain reaction results revealed the stable integration of the transgene in the progeny of the engineered plants. The transgenic plants with increased vitamin C content in the fruit showed enhanced tolerance to abiotic stresses induced by methyl viologen, NaCl, and mannitol as compared with wild-type plants. The leaf disc senescence assay showed better tolerance in transgenic plants by retaining higher chlorophyll content compared with wild-type plants. The T1 plants survived under salt stress (200 mM NaCl) whereas wild-type plants were unable to survive such high salt concentrations. This study suggests that the increased accumulation of AsA could upregulate the antioxidant system which imparts improved tolerance against various abiotic stresses in transgenic tomato plants as compared with wild-type plants.
机译：维生素C（抗坏血酸，AsA）是胶原生物合成的必需成分，也是人类心血管系统的正常功能。植物和一些动物可以合成抗坏血酸，而人类缺乏抗坏血酸生物合成必需的基因，必须从饮食中获取维生素。在本研究中，我们开发了一种过表达由CaMV35S组成型启动子驱动的大鼠1-gulono-γ-内酯氧化酶（GLOase）基因的转基因番茄植物（Lycopersicon esculentum Mill。）。 GLOase的过表达导致转基因番茄果实中AsA含量增加1.5倍，这与GLOase活性增加呈正相关。 Southern和逆转录酶聚合酶链反应结果表明转基因在工程植物后代中的稳定整合。与野生型植物相比，果实中维生素C含量增加的转基因植物显示出对甲基紫精，NaCl和甘露醇诱导的非生物胁迫的耐受性增强。与野生型植物相比，叶盘衰老测定法通过保留更高的叶绿素含量在转基因植物中显示出更好的耐受性。 T1植物在盐胁迫（200 mM NaCl）下存活，而野生型植物无法在如此高的盐浓度下存活。这项研究表明，与野生型植物相比，AsA积累的增加可以上调抗氧化系统，从而提高转基因番茄植物对各种非生物胁迫的耐受性。

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