...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >In vitro propagation of purple pitahaya (Hylocereus costaricensis [FAC Weber] Britton & Rose) cv. Cebra
【24h】

In vitro propagation of purple pitahaya (Hylocereus costaricensis [FAC Weber] Britton & Rose) cv. Cebra

机译：紫色火龙果（Hylocereus costaricensis [FAC Weber] Britton＆Rose）的体外繁殖。塞布拉

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Limitations to large-scale propagation of purple pitahaya (Hylocereus costaricensis [F.A.C. Weber] Britton & Rose), a potential source of betalains for the food industry, can be overcome through utilization of in vitro culture technologies. In this work, successful in vitro propagation from areoles of adult purple pitahaya plants is reported. Factors affecting culture initiation, bud sprouting and growth, shoot multiplication, rooting, and acclimatization were studied. Best results for culture initiation were obtained from the central region of new joints by disinfection of large (5–7 cm in length) explants that were subsequently divided. Explants were sequentially treated with Extran? alkaline detergent for 10 min, followed by immersion in 70 % (v/v) ethanol for 15–30 s, a mixture of the fungicide Benomyl and the bactericide Agrymicin (2 g?l?1 each) for 30 min, and disinfection in sodium hypochlorite (1.0 %?w/v) for 15 min. Culture of sectioned individual areoles, without removing thorns, on Murashige and Skoog medium supplemented with 15 or 30 μM N 6-benzylaminopurine (BAP) for 3 mo with monthly subcultures, followed by transfer to the same medium with reduced BAP (0–2 μM), induced bud sprouting in over 80 % of explants, adequate growth of the shoots, with production of lateral shoots, and spontaneous rooting within 160 d. These plants were successfully acclimatized in vermiculite and peat moss (1:1), or perlite and peat moss (2:1) in the greenhouse, with over 90 % survival rate. One hundred percent of the in vitro-derived plants were successfully transferred to the field. Furthermore, these plants showed higher survival rates, larger height and increase in stem diameter than equivalent plants from the same genotype, derived from stem segments (the common clonal propagation system utilized for this species) that were simultaneously planted.
机译：可以通过使用体外培养技术来克服紫色火龙果（Hylocereus costaricensis [F.A.C. Weber] Britton＆Rose）大规模繁殖的局限性，而后者是食品工业中甜菜碱的潜在来源。在这项工作中，报道了从成年紫色火棘植物的甲虫成功的体外繁殖。研究了影响培养起始，芽萌芽和生长，枝条繁殖，生根和驯化的因素。通过对大型（长度为5-7 cm）外植体进行消毒，然后将其分离，可以从新关节的中央区域获得培养开始的最佳结果。外植体依次用Extran？碱性洗涤剂中浸泡10分钟，然后将其浸入70％（v / v）乙醇中15-30 s，杀菌剂苯菌灵和杀菌剂阿格霉素的混合物（各2 g？l？1）浸泡30分钟，并在次氯酸钠（1.0％w / v）15分钟。在补充了15或30μMN 6-苄基氨基嘌呤（BAP）的Murashige和Skoog培养基上进行3个月的逐日分离培养，将无须刺的切成小孔，然后逐次培养至BAP降低（0–2μM ），导致超过80％的外植体发芽，芽充分生长，产生侧生芽，并在160 d内自发生根。这些植物已成功在温室中的ver石和泥炭藓（1：1）或珍珠岩和泥炭藓（2：1）中适应，存活率超过90％。 100％的体外植物成功转移到田间。而且，与同时种植的茎段（用于该物种的普通克隆繁殖系统）衍生的相同基因型的同等植物相比，这些植物显示出更高的成活率，更大的高度和茎直径的增加。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号