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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >In vitro regeneration of wild chervil (Anthriscus sylvestris L.).
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In vitro regeneration of wild chervil (Anthriscus sylvestris L.).

机译：野生山vil（ Ansrisris sylvestris L.）的体外再生。

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摘要

Anthriscus sylvestris (L.) Hoffm. (Apiaceae) is a common wild plant that accumulates the lignan deoxypodophyllotoxin. Deoxypodophyllotoxin can be hydroxylated at the C-7 position in recombinant organisms yielding podophyllotoxin, which is used as a semi-synthetic precursor for the anticancer drugs, etoposide phosphate and teniposide. As in vitro regeneration of A. sylvestris has not yet been reported, development of a regeneration protocol for A. sylvestris would be useful as a micropropagation tool and for metabolic engineering of the plant. Calli were induced from hypocotyl explants and transferred to shoot induction medium containing zeatin riboside. Regenerated shoots were obtained within 6 mo and were transferred onto growth regulator-free root induction medium containing 1% sucrose. Regenerated plants transferred to soil and acclimatized in a greenhouse. Plants were transferred to the field with a 100% survival rate. Regenerated plants flowered and were fully fertile. This is the first report of complete regeneration of A. sylvestris via shoot organogenesis from callus.
机译：樟子松（L.）Hoffm。 （伞形科）是一种常见的野生植物，会积聚木脂素脱氧鬼臼毒素。脱氧鬼臼毒素可以在重组生物的C-7位置羟基化，生成鬼臼毒素，鬼臼毒素用作抗癌药，依托泊苷磷酸酯和替尼泊苷的半合成前体。作为 A的体外再生。樟子松尚未有报道， A的再生方案的发展。樟子松可用作微繁殖工具和植物的代谢工程。从下胚轴外植体诱导愈伤组织，并将其转移至含有玉米素核糖苷的芽诱导培养基中。在6mo内获得再生的芽，并将其转移到含有1％蔗糖的无生长调节剂的根诱导培养基上。再生植物转移到土壤中并在温室中适应环境。将植物以100％的存活率转移到田间。再生的植物开花并完全肥沃。这是 A完全再生的第一份报告。 call通过愈伤组织的芽器官发生。

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