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首页> 外文期刊>Indian Journal of Biotechnology >Iron induced modifications in physiological attributes and SDS-PAGE of whole cell proteins pattern of Anabaena PCC 7120 and its derivative ntcA mutant
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Iron induced modifications in physiological attributes and SDS-PAGE of whole cell proteins pattern of Anabaena PCC 7120 and its derivative ntcA mutant

机译：铁诱导的鱼腥藻PCC 7120及其衍生物ntcA突变体的生理特性修饰和全细胞蛋白质模式的SDS-PAGE

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摘要

The influence of the various levels of iron (0-500 mu M) on Anabaena sp. PCC 7120 and its derivative CSE2 mutant impaired in ntcA gene have been investigated to determine the requirement of specific concentration of iron for the regulation of photosynthetic pigments, cellular constituents, heterocyst spacing pattern, enzyme activities and SDS-PAGE of whole cell protein profiling. Iron at lower concentrations (<= 50 mu M) causes increase in chlorophyll a, carotenoid, phycocyanin, carbohydrate, protein, nitrogenase, nitrate reductase and glutamine synthetase activities in wild type as well as in mutant strain (except nitrogenase and nitrate reductase activities), while higher concentrations (>50 mu M) were toxic and cause significant reductions in these physiological attributes. Nitrogenase and nitrate reductase activity were found absent in ntcA mutant. Further, 50 mu M iron concentration also supported the maximum heterocyst frequency with changed heterocyst spacing pattern in wild type, whereas no visible sign of heterocyst development was observed in mutant strain. SDS-PAGE of whole cell protein revealed the occurrence of maximum number of bands at 50 mu M iron concentration in both wild type and mutant strain. Results suggested that 50 mu M of iron concentration was the specific concentration (critical concentration) that supported optimal physiological and biochemical activities in Anabaena 7120 and its mutant strain CSE2 (ntcA).
机译：不同水平的铁（0-500微米）对鱼腥藻的影响。已经研究了在ntcA基因中受损的PCC 7120及其衍生的CSE2突变体，以确定铁的特定浓度对调节光合色素，细胞成分，异胚胞间隔模式，酶活性和全细胞蛋白谱分析的SDS-PAGE的要求。较低浓度（<= 50μM）的铁导致野生型以及突变菌株中的叶绿素a，类胡萝卜素，藻蓝蛋白，碳水化合物，蛋白质，固氮酶，硝酸还原酶和谷氨酰胺合成酶活性增加（固氮酶和硝酸还原酶活性除外） ，而更高的浓度（> 50μM）则具有毒性，并导致这些生理特性的显着降低。发现ntcA突变体中不存在氮酶和硝酸还原酶活性。此外，在野生型中50μM的铁浓度还支持了最大的异性囊频率，改变了异性囊的间隔模式，而在突变菌株中没有观察到异性囊发育的迹象。全细胞蛋白的SDS-PAGE揭示了在野生型和突变株中，在50μM铁浓度下最大条带的出现。结果表明，50μM铁浓度是支持Anabaena 7120及其突变菌株CSE2（ntcA）最佳生理和生化活性的特定浓度（临界浓度）。

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