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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Micropropagation of Eucalyptus benthamii to form a clonal micro-garden
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Micropropagation of Eucalyptus benthamii to form a clonal micro-garden

机译：桉树微繁殖形成无性系微花园

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摘要

Eucalyptus benthamii is an important component of forestry plantations in cold regions, but it is difficult to obtain clonal plants of this species, especially by low rooting. In this study, we developed a method for cloning selected genotypes of E. benthamii using a micropropagation technique, enabling the formation of a clonal micro-garden. Nodal segments from sprouts of mini-stumps in the clonal mini-garden were used as explants. After in vitro establishment of the explants, we tested two selected clones (BP101 and BP118), three culture media (Wood Plant Medium (WPM), Correia and colleagues JADS medium, and Murashige and Skoog medium), and two plant growth regulators (6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA)) for the multiplication of adventitious buds. Additionally, combinations of two other plant growth regulators (BAP and gibberellic acid (GA3)) were tested for the elongation of shoots. The in vitro and ex vitro rooting of micro-plantlets prior to acclimatization were compared. The in vitro bud multiplication of E. benthamii depended on the clone, culture medium, and concentration of plant growth regulators. The best results were obtained with WPM supplemented with 0.5 mg L?1 BAP and 0.05 mg L?1 NAA. The elongation of shoots depended on the clone and plant growth regulator, and the best results were obtained with nutrient medium free of GA3 and BAP. Histological analysis showed that both in vitro and ex vitro rooting were successful, resulting in normal development of adventitious roots showing a vascular connection with the vascular cambium. The new protocol is efficient for micro-plantlet production of E. benthamii and can be used for the formation of a clonal micro-garden for other Eucalyptus or tree species.
机译：寒地桉树是寒冷地区林业人工林的重要组成部分，但很难获得该物种的无性系植物，尤其是通过低生根。在这项研究中，我们开发了一种使用微繁技术克隆本氏大肠杆菌的选定基因型的方法，从而能够形成克隆的微花园。来自克隆迷你花园中迷你树桩的芽的节段用作外植体。在体外建立外植体后，我们测试了两个选定的克隆（BP101和BP118），三种培养基（木材植物培养基（WPM），Correia和他的同事JADS培养基以及Murashige和Skoog培养基）和两个植物生长调节剂（6 -苄基氨基嘌呤（BAP）和α-萘乙酸（NAA））用于不定芽的繁殖。此外，还测试了两种其他植物生长调节剂（BAP和赤霉素（GA3））的组合，用于枝条的伸长。比较了适应之前微植株的体外和离体生根。本氏肠球菌的体外芽繁殖取决于克隆，培养基和植物生长调节剂的浓度。补充0.5 mg L？1 BAP和0.05 mg L？1 NAA的WPM可获得最佳结果。枝条的伸长取决于克隆和植物生长调节剂，并且在不含GA3和BAP的营养培养基上可获得最佳结果。组织学分析表明，体外和离体生根均成功，导致不定根的正常发育，显示出与血管形成层之间存在血管连接。该新协议对于本氏小肠埃希氏菌的微植株生产是有效的，并可用于形成其他桉树或树木物种的克隆微花园。

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