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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Adventitious shoot regeneration from ovaries of Hosta 'Golden Scepter'.
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Adventitious shoot regeneration from ovaries of Hosta 'Golden Scepter'.

机译：玉Host“金权杖”卵巢的不定芽再生。

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摘要

The objective of this study was to evaluate the ability of Hosta cv. Golden Scepter (GS) ovary explants to generate adventitious shoots in vitro. Ovaries were transversely cut into halves and transferred to petri dishes containing Hosta initiation medium supplemented with NAA at 2.5 鍹 and N6-benzyladenine (BA) at 10 鍹. GS produced adventitious shoots from the ovary base via organogenesis. The number of adventitious shoots regenerated from callus increased linearly with repeated subculturing on Murashige and Skoog (MS) medium supplemented with 2.5 鍹 NAA and 10 鍹 BA. Multiple shoots developed from callus (15.8), shoot tip (8.4), leaf (6.7), and root (4.3) explants on MS medium supplemented with 2.5 鍹 NAA and 20-30 鍹 BA. There were significantincreases in the number of shoots regenerated from shoot tips and callus on MS medium with 50 and 100 mg myo-inositol/litre. Similarly, there were significant increases in the number of axillary shoots and adventitious shoots produced with 20 g sucrose/litre.
机译：这项研究的目的是评估玉a的能力。金色权杖（GS）卵巢外植体在体外产生不定芽。将卵巢横向切成两半，并转移到含有添加有2.5 pet NAA和10鍹N6-苄腺嘌呤（BA）的玉Host属起始培养基的培养皿中。 GS通过器官发生从卵巢基部产生不定芽。从愈伤组织再生的不定芽的数量随着在补充有2.5鍹NAA和10鍹BA的Murashige和Skoog（MS）培养基上反复传代而线性增加。在补充有2.5鍹NAA和20-30鍹BA的MS培养基上，从愈伤组织（15.8），芽尖（8.4），叶（6.7）和根（4.3）外植体形成了许多芽。在50和100 mg肌醇/升的MS培养基上，从芽尖和愈伤组织再生的芽数显着增加。同样，以20克蔗糖/升生产的腋生芽和不定芽的数量也有显着增加。

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