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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Genetic transformation mediated by Agrobacterium tumefaciens of florists' chrysanthemum (Dendranthema Xgrandiflorum) cultivar 'Peach Margaret'.
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Genetic transformation mediated by Agrobacterium tumefaciens of florists' chrysanthemum (Dendranthema Xgrandiflorum) cultivar 'Peach Margaret'.

机译：由花农菊花（Dendranthema Xgrandiflorum）品种“桃玛格丽特”的根癌农杆菌介导的遗传转化。

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摘要

A genetic transformation method using A. tumefaciens strain LBA4404 and based on the neomycin phosphotransferase II (nptII) selectable marker gene is described for D. grandiflorum [D. morifolium] cv. Peach Margaret. The flavonoid regulatory cDNA Lc(Leaf colour) from Zea mays, under control of the cauliflower mosaic caulimovirus 35S promoter, was used to test if it could serve as a visible, non-destructive pigmentation reporter of transformation in chrysanthemum and to see if it would modify floraland leaf pigmentation. Stable integration of the maize Lc cDNA in Peach Margaret was confirmed by Southern DNA analysis. However, no Lc RNA transcripts were detected and no increase in pigmentation was observed in the transformants. In contrast, activity of the nptII transgene in the transformants was confirmed by production of roots in the presence of 20 mg kanamycin/litre and challenging leaf explants to regenerate shoots in the presence of 25 mg kanamycin/litre. This is the first reported method based on A. tumefaciens for genetic transformation of cv. Peach Margaret.
机译：针对桔梗的描述了一种使用根癌农杆菌LBA4404并基于新霉素磷酸转移酶II（nptII）选择标记基因的遗传转化方法。 rif] cv。桃玛格丽特。在花椰菜花椰菜花椰菜花叶病毒35S启动子的控制下，来自玉米的黄酮调节性cDNA Lc（叶色）被用于测试它是否可以充当菊花转化中可见的，无损的色素沉着报道分子，并观察是否改变花和叶的色素沉着。通过Southern DNA分析证实玉米Lc cDNA在桃玛格丽特中的稳定整合。然而，在转化体中未检测到Lc RNA转录物，也未观察到色素沉着的增加。相反，通过在20mg卡那霉素/升的存在下产生根和在25mg卡那霉素/升的存在下挑战叶外植体再生枝来证实转化体中nptII转基因的活性。这是首次报道的基于根癌农杆菌的cv遗传转化方法。桃玛格丽特。

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