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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Effects of auxin and light treatments of donor plants on shoot production from indica-type rice (Oryza sativa L.).
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Effects of auxin and light treatments of donor plants on shoot production from indica-type rice (Oryza sativa L.).

机译:生长素和供体光处理对in型水稻(Oryza sativa L.)芽的生产的影响。

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摘要

An efficient clonal propagation procedure for a Brazilian indica rice subspecies was developed with shoot apex explants. Shoot apices were excised from 4-day-old seedlings and cultured on MS medium supplemented with 8.9 鍹 6-benzyladenine. The efficiency of shoot production was influenced by growth regulators and light treatments to the donor plant. Explants derived from seedlings grown in the presence of 10.7 鍹 NAA and in the absence of light showed significantly increased regeneration capacity compared with control explants. Anatomical analysis of the new shoot meristems revealed that they originated from pre-existing apical and axillary meristem as well as from the mesocotyl parenchyma.
机译:开发了巴西shoot稻亚种的高效克隆繁殖程序,并带有芽尖外植体。从4天大的幼苗上切下茎尖,并在补充有8.9鍹6-苄基腺嘌呤的MS培养基上培养。芽生产的效率受生长调节剂和供体植物的光照处理的影响。与对照外植体相比,在10.7鍹NAA存在下且在无光照条件下生长的幼苗衍生的外植体显示出显着提高的再生能力。对新芽分生组织的解剖学分析表明,它们起源于既存的​​根尖和腋生分生组织以及中胚轴薄壁组织。

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