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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Influence of bombardment with BA-coated microprojectiles on shoot organogenesis from Phlox paniculata L. and Pinus pinea L. tissues.
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Influence of bombardment with BA-coated microprojectiles on shoot organogenesis from Phlox paniculata L. and Pinus pinea L. tissues.

机译：包被BA的微粒轰击对来自福禄考（Plox paniculata L.）和松（Pinus pinea L.）组织的芽器官发生的影响。

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摘要

Leaves of Phlox paniculata were bombarded with benzyladenine (BA)-coated gold microprojectiles with the Biolistic PDS/1000-He gene gun. Increasing the concentration of BA from 0.01 to 0.1 mg/鎙 increased the mean number of adventitious shoots from 7to 16 shoots per leaf. Bombardment of cotyledons of Pinus pinea (Italian stone pine) with BA-coated gold particles significantly reduced the frequency of shoot organogenesis by about 40%. However, induced shoot regenerants from bombarded explants of both P. paniculata and P. pinea were longer (>1 cm) than those derived from control explants after 3 and 5 weeks following bombardment, respectively.
机译：用Biolistic PDS / 1000-He基因枪用苄基腺嘌呤（BA）包裹的金微粒轰击福禄考的叶子。将BA的浓度从0.01增至0.1 mg / l，可使不定梢的平均数量从每片7至16个芽增加。用BA包被的金颗粒轰击松树（意大利松树）的子叶，可使芽器官发生的频率显着降低约40％。然而，分别由P. paniculata和P. pinea轰击的外植体诱导的嫩芽再生长（> 1 cm），分别比轰击后3周和5周来自对照外植体的诱导再生长。

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