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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Studies on stomatal function, epicuticular wax and stem-root transition region of polyethylene glycol-treated and nontreated in vitro grape plantlets.
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Studies on stomatal function, epicuticular wax and stem-root transition region of polyethylene glycol-treated and nontreated in vitro grape plantlets.

机译：聚乙二醇处理和未处理的体外葡萄苗的气孔功能，表皮蜡和茎根过渡区的研究。

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摘要

Scanning electron microscopy (SE), light microscopy and gravimetric analysis were used to evaluate stomata function, epicuticular wax and the stem-root transition region of grape cv. Valiant plantlets (grown in vitro or in a greenhouse). SE studiesof leaf surfaces of in vitro-cultured and greenhouse-grown plants showed widely open stomata compared with the leaf stomata of polyethylene glycol (PEG)-treated in vitro-cultured and greenhouse-grown plants. Ultrastructurally, leaf epicuticular wax of invitro-plants was less dense than in their PEG-treated and greenhouse counterparts. Quantitatively, in vitro-grown plants had reduced epicuticular wax compared with PEG-treated and greenhouse-grown plants. Light microscopic studies showed no obvious differences in the vascular connections in the stem-root transition region of in vitro-cultured, PEG-treated in vitro-cultured, and greenhouse-grown plants. It was therefore likely that the rapid wilting and desiccation observed after transplanting in vitrogrape plantlets was due to their defective stomatal function and reduced epicuticular wax and may not be due to poor water transport associated with vascular connections.
机译：扫描电子显微镜（SE），光学显微镜和重量分析法用于评估葡萄cv的气孔功能，表皮蜡和茎根过渡区。雄壮的小植株（在体外或温室中生长）。与聚乙二醇（PEG）处理的离体栽培和温室栽培植物的叶片气孔相比，离体栽培和温室栽培植物的叶片表面的SE研究显示出宽阔的气孔。在超微结构上，离体植物的叶片表皮蜡比在PEG处理的和温室的对应物中密度较小。从数量上讲，与PEG处理和温室种植的植物相比，体外种植的植物减少了表皮蜡。光学显微镜研究表明，在体外培养，PEG处理的体外培养植物和温室种植植物的茎根过渡区中，维管束连接没有明显差异。因此，很可能是在移栽试管葡萄苗后观察到的快速萎des和干燥是由于它们的气孔功能不良和表皮蜡减少，而不是由于与血管连接有关的不良水传输。

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