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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Changes in distribution of zeatin and indole-3-acetic acid in cells during callus induction and organogenesis in vitro in immature embryo culture of wheat
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Changes in distribution of zeatin and indole-3-acetic acid in cells during callus induction and organogenesis in vitro in immature embryo culture of wheat

机译：小麦未成熟胚培养过程中愈伤组织诱导和器官发生过程中玉米醇溶蛋白和吲哚-3-乙酸的分布变化

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Plant organogenesis remains one of the most essential questions of plant developmental biology. Callus tissue in vitro is a valuable tool for the studies on hormonal aspects of plant organogenesis, especially its early events, and immunohistochemical analysis is one of the few approaches offering information on the localization and role of hormones during organ development. The localization of endogenous zeatin and indole-3-acetic acid was investigated during simultaneous bud and root formation in calluses derived from immature embryos of wheat (Triticum aestivum L.). Calluses were induced on Murashige and Skoog (MS) medium supplemented with 2.0 mg L-1 2,4-dichlorophenoxyacetic acid. To stimulate simultaneous bud and root formation, calluses were transferred onto MS medium supplemented with 0.2 mg L-1 kinetin and 0.2 mg L-1 indoleacetic acid. Strong immunostaining for both hormones was detected in proliferating callus tissue, in developing meristematic centers and meristematic zones (whose cells were shown to be involved in organ formation), and at the sites of shoot and root apex initiation. During further development, shoot apexes with leaf primordia were heavily immunostained for zeatin, while immunostaining for indole-3-acetic acid was more intense at the sites of leaf primordia initiation and incipient primordia themselves. In the developing roots, immunostaining for both hormones reached a maximum in the root apex and gradually declined with increasing distance from the apex. Cells of developing procambial strands were also strongly stained for both zeatin and indole-3-acetic acid. These data suggest considerable similarity between patterns of hormone distribution in organs in vitro and in vivo. Thus, callus culture is a convenient and useful model for the study of fundamental biological questions such as how hormones regulate development.
机译：植物器官发生仍然是植物发育生物学最重要的问题之一。体外愈伤组织是研究植物器官发生的激素方面特别是其早期事件的有价值的工具，免疫组织化学分析是提供有关器官发育过程中激素的定位和作用的信息的少数方法之一。内源玉米蛋白和吲哚-3-乙酸的定位是在小麦和小麦幼胚的愈伤组织中同时芽和根形成过程中进行的。在补充有2.0 mg L-1 2,4-二氯苯氧基乙酸的Murashige和Skoog（MS）培养基上诱导愈伤组织。为了刺激芽和根同时形成，将愈伤组织转移至补充有0.2 mg L-1激动素和0.2 mg L-1吲哚乙酸的MS培养基上。在增生的愈伤组织，发育中的分生中心和分生区（显示其细胞参与器官形成）以及芽和根尖的起始部位均检测到了两种激素的强免疫染色。在进一步的发育过程中，带有叶原基的茎尖对玉米蛋白的免疫染色严重，而吲哚-3-乙酸的免疫染色在叶原基和起始原基本身的位点更强烈。在发育中的根中，两种激素的免疫染色在根尖达到最大，并随着距根距离的增加而逐渐降低。发育中的procambian链的细胞也强烈染色了玉米蛋白和吲哚-3-乙酸。这些数据表明在体内和体外器官中激素分布模式之间有相当大的相似性。因此，愈伤组织培养是研究基本生物学问题（如激素如何调节发育）的方便且有用的模型。

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