Experiments were undertaken to determine the survival of indicator and pathogenic bacteria in samples of a popular orange soft drink having pH 3.0 and held at 8 degrees C. The fizzy orange drink was inoculated with c. 10(6) cell ml(-1) of Escherichia coli (two strains, EAC1 and EAC75) and c. 10(6) cell ml(-1) of Salmonella derby (two strains) and Listeria monocytogenes (one strain). None of the strains of S. derby and L. monocytogenes was detected 35 h after inoculation. Numbers of the E. coli decreased, reaching a final concentration of 1.1 x 10(2) CFU ml(-1) and 1.3 x 10(3) CFU ml(-1) for strain EAC1 and strain EAC75, respectively. These results indicate the safety of this type of soft drink with respect to these organisms.
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