Comparison of the partial 16S rRNA gene sequences and development of oligonucleotide probes for the detection of Escherichia coli cells in water and milk

Lin CK; Tsen HY

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Comparison of the partial 16S rRNA gene sequences and development of oligonucleotide probes for the detection of Escherichia coli cells in water and milk

机译：Comparison of the partial 16S rRNA gene sequences and development of oligonucleotide probes for the detection of Escherichia coli cells in water and milk

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The primary sequence of the V3 and V6 regions of the 16S ribosomal RNA genes of non-pathogenic and pathogenic E. coli strains were determined and compared with those of non-E. coli strains, to develop E. coli-specific DNA probes. Results showed thatall the E. coli strains tested generated positive hybridization signals, while none of the non-E. coli strains gave a false-positive result. The probes could be used to detect levels of E. coli as low as 10 c.f.u./100 ml in tap water or milk.

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《Food Microbiology》 |1999年第6期|551-562|共12页
作者
Lin CK; Tsen HY;
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收录信息美国《科学引文索引》(SCI);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类食品工业;
关键词
milk; water; microbial contamination; DNA probes; DNA hybridization; analytical methods; pathogens; ribosomal RNA; milk hygiene; drinking water; bacterial diseases; Escherichia coli;

Comparison of the partial 16S rRNA gene sequences and development of oligonucleotide probes for the detection of Escherichia coli cells in water and milk

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