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首页> 外文期刊>Indian Journal of Experimental Biology >Mouse acquired HPV tumor using dorsal skin-fold window chamber
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Mouse acquired HPV tumor using dorsal skin-fold window chamber

机译：小鼠使用背部皮肤折叠窗腔室获得HPV肿瘤

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摘要

Human papillomavirus (HPV) plays important role in developing several types of cancer. In order to understand the viral pathogenesis, the animal model of HPV infection is very necessary. This communication reports establishment of an animal model carrying implanted HeLa cells, a human cervical cancer cell line via dorsal skin-fold window chambers. Nude mice were divided into 4 groups each contained different amount of Hela cells. 2.5x10(5), 5x10(5), and 1x10(6) cells, and cell free medium (control), respectively. The results showed that even using the low number of HeLa cells (2.5x10(5)), the tumor microvasculature was developed at 2 weeks after implantation with the enlarged tumor margin which then progressed to tumor mass in the following week. The existing tumor was confirmed to be HeLa-cell type by PCR in situ hybridization, and HPV genotyping. By using linear regression analysis, it indicated that means of tumor size from each group significantly increased in relation to number of HeLa cells used (R-2 = 0.98, y = 0.1171x+4.35). This mouse model will be useful for the HPV studies particularly anti-cancer drugs efficacy.
机译：人乳头瘤病毒（HPV）在发展几种类型的癌症中起着重要作用。为了了解病毒的发病机理，HPV感染的动物模型是非常必要的。该来文报道建立了一个通过背部皮肤折叠窗口腔室携带植入的HeLa细胞（一种人类宫颈癌细胞系）的动物模型。裸鼠分为4组，每组包含不同数量的Hela细胞。 2.5x10（5），5x10（5）和1x10（6）单元格以及无细胞培养基（对照）。结果表明，即使使用少量的HeLa细胞（2.5x10（5）），在植入后2周时肿瘤微脉管系统也会发育，肿瘤边缘增大，然后在接下来的一周内发展为肿瘤块。通过PCR原位杂交和HPV基因分型证实存在的肿瘤为HeLa细胞类型。通过线性回归分析，表明相对于使用的HeLa细胞数量，每组的肿瘤大小均值均显着增加（R-2 = 0.98，y = 0.1171x + 4.35）。该小鼠模型将对HPV研究特别是抗癌药功效有用。

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