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首页> 外文期刊>Indian Journal of Fisheries >Development of expressed sequence tags (ESTs) from the brain tissue of snowtrout Schizothorax richardsonii (Gray, 1832) (Family Cyprinidae) and its preliminary annotation
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Development of expressed sequence tags (ESTs) from the brain tissue of snowtrout Schizothorax richardsonii (Gray, 1832) (Family Cyprinidae) and its preliminary annotation

机译：雪蛤Schizothorax richardsonii（灰色，1832年）（鲤科）的脑组织表达序列标签（EST）的开发

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The snowtrout (Schizothorax richardsonii) is an economically important fish in the north-east Himalayan region. However, genomic research on this species is still in its infancy, and genomic resources are largely unavailable. The objective of this study was to generate expressed sequence tags (ESTs) from a brain complementary DNA (cDNA) library and to identify the genes. A total of 1031 ESTs were sequenced, from which 73 contigs and 411 singletons were identified. BLAST homology analysis indicated that only 9.3% of these ESTs were homologues of known genes while the remaining 90.7% appeared to be novel sequences. Based on sequence similarities, 45 putative genes were identified that encodes stress proteins, enzymes and signal transduction regulators. Our study thus, provides a collection of novel transcripts and a partial annotation of genes that are expressed in brain tissue of the snowtrout species, S. richardsonii
机译：鳟鱼（Schizothorax richardsonii）是喜马拉雅东北部的重要经济鱼类。然而，对该物种的基因组研究仍处于起步阶段，并且基因组资源基本上不可用。这项研究的目的是从大脑互补DNA（cDNA）文库生成表达的序列标签（EST）并鉴定基因。总共测序了1031个EST，从中鉴定出73个重叠群和411个单例。 BLAST同源性分析表明，这些EST中只有9.3％是已知基因的同源物，而其余的90.7％似乎是新序列。根据序列相似性，鉴定了45个推定基因，它们编码胁迫蛋白，酶和信号转导调节剂。因此，我们的研究提供了一组新的转录本和部分注释的基因，这些基因在雪鳟物种S. richardsonii的脑组织中表达

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