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首页> 外文期刊>Indian Journal of Fisheries >Effect of water temperature on haemocyte responses in the disk abalone Haliotis discus hannai Ino, 1953
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Effect of water temperature on haemocyte responses in the disk abalone Haliotis discus hannai Ino, 1953

机译：水温对圆盘鲍鱼Haiotis discus hannai Ino，1953年血细胞反应的影响

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摘要

Effects of gradual water temperature modulations on neutral red retention time (NRR time) of lysosomes in haemocytes and haemocyte counts were studied in the disk abalone Haliotis discus hannai Ino, 1953. Experimental animals were acclimated at 18 degrees C for a period of one week before exposing to gradual water temperature modulations. After acclimation, the water temperature was gradually increased/decreased @ 1 degrees C every other day until the target temperature of 10 degrees C/26 degrees C was reached. The temperatures were maintained for seven days before being brought back to 18 degrees C from 10 degrees C/26 degrees C (@ 1 degrees C per day). Haemolymph samples were collected 48 h after each temperature change. The haemocyte density at 26 degrees C was significantly higher (p<0.05) than that at 18 degrees C and 10 degrees C, and the lowest haemocyte density was obtained at 10 degrees C. The NRR time of the abalone held at 26 degrees C, 18 degrees C and 10 degrees C were 31.6 +/- 1.1min, 84.6 +/- 2.7 min and 41.3 +/- 1.7 min, respectively. The temperature changes between 17 degrees C and 20 degrees C did not significantly affect the NRR time suggesting that this is the optimal water temperature range for maintaining lysosomal membrane integrity in this species.
机译：在盘状鲍鱼Haliotis discus hannai Ino，1953年研究了逐渐调节水温对溶酶体中溶酶体的中性红保留时间（NRR时间）和血细胞计数的影响。实验动物在18摄氏度下适应环境，一周后暴露于逐渐的水温调节。适应后，水温每隔一天在1摄氏度下逐渐升高/降低，直到达到10摄氏度/ 26摄氏度的目标温度。将温度保持7天，然后从10摄氏度/ 26摄氏度（每天1摄氏度）恢复到18摄氏度。每次温度变化48小时后收集血淋巴样品。 26摄氏度时的血细胞密度显着高于18摄氏度和10摄氏度时的（p <0.05），而10摄氏度时的血细胞密度最低。鲍鱼在26摄氏度时的NRR时间保持不变， 18摄氏度和10摄氏度分别为31.6 +/- 1.1分钟，84.6 +/- 2.7分钟和41.3 +/- 1.7分钟。 17摄氏度至20摄氏度之间的温度变化不会显着影响NRR时间，这表明这是维持该物种溶酶体膜完整性的最佳水温范围。

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