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首页> 外文期刊>Indian Journal of Fisheries >Identification of brown mussel (Perna indica) larvae using molecular tool
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Identification of brown mussel (Perna indica) larvae using molecular tool

机译：分子工具鉴定褐贻贝幼虫

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摘要

Species identification of larvae is crucial in understanding larval distribution and population ecology of bivalves. There is great prospects for brown mussel (Perna indica) mariculture and therefore the natural spat collection as a source of seed for culture gains importance. It is critical to precisely identify the larvae of the species in order to predict the spat fall for successful spat collection. The larvae of different bivalve species have similar morphology which makes it difficult to identify the species by microscopy. The partial coding sequences of the mitochondrial gene, cytochrome oxidase c subunit 1 (CO1) of P. indica and other related bivalve species were compared and a pair of species specific PCR (SSPCR) primers was designed that can specifically amplify the DNA of P. indica. The assay conducted to establish the specificity of SSPCR primers produced 291 bp PCR product, only with the target species. The SSPCR conducted with individual veliger larva sorted from the field collected plankton samples have confirmed the presence of P. indica. The SSPCR with the whole plankton samples showed presence of the species from the last week of May until the last week of June 2011.
机译：幼虫的物种鉴定对于了解双壳虫的幼虫分布和种群生态学至关重要。褐贻贝（印度Perna）海水养殖有广阔的前景，因此，作为养殖种子来源的天然natural收集变得越来越重要。准确地识别物种的幼虫，以预测成功落下的鱼卵至关重要。不同双壳类物种的幼虫具有相似的形态，这使得很难通过显微镜鉴定该物种。比较了印度假单胞菌和其他相关双壳类物种的线粒体基因，细胞色素氧化酶c亚基1（CO1）的部分编码序列，并设计了一对物种特异性PCR（SSPCR）引物，可以特异性扩增P. 。为确定SSPCR引物的特异性而进行的测定仅产生了目标物种，产生了291 bp PCR产物。用从田间采集的浮游生物样品中分选出的单个虫幼虫进行的SSPCR证实了印度。草的存在。从整个浮游生物样本进行的SSPCR显示，从2011年5月的最后一个星期到2011年6月的最后一个星期存在该物种。

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