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Development of an image system for the study of viable but non-culturable forms of Campylobacter jejuni and its use to determine their resistance to disinfectants

机译:Development of an image system for the study of viable but non-culturable forms of Campylobacter jejuni and its use to determine their resistance to disinfectants

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摘要

A vital staining method incorporating fluorescence microscopy and image analysIs has been developed to study the viable but non-culturable (VNC) form of Campylobacter jejuni. This method was used to determine the comparative resistance of the culturable and VNC forms to proprietary disinfectants chosen to represent the four main groups of disinfectants used in food processing operations, namely quaternary ammonium compounds (QACs) and QACs combined with glutaraldehyde, amphoterics and chlorine. With all disinfectants except chlorine the culturable form was significantly more resistant than the VNC form. The resistance of the VNC form to chlorine may have significance for cleaning and decontamination in the food industry.

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