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首页> 外文期刊>Indian Journal of Small Ruminants >Cultural isolation and PCR-based identification of Bacillus anthracis isolates from western region of India.
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Cultural isolation and PCR-based identification of Bacillus anthracis isolates from western region of India.

机译：来自印度西部地区的炭疽芽孢杆菌分离株的文化分离和基于PCR的鉴定。

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摘要

In the present study, Bacillus anthracis was isolated on blood agar from post-mortem samples collected from two different outbreaks at one year interval in sheep and goat flocks of western region of India. The identities of the isolates were confirmed by conventional cultural techniques and Polymerase Chain Reaction (PCR). Products of expected size were amplified after PCR using specific primers targeting cya gene, capsule gene, S-layer chromosomal region and a B. anthracis specific chromosomal marker - Ba813 (929, 846, 639 and 168 bp, respectively), which confirmed the identity of the isolates and also underlines the significance of this molecular technique in the rapid and specific diagnosis of potentially zoonotic infectious agent in comparison to conventional culture techniques.
机译：在本研究中，炭疽杆菌是从印度西部地区的绵羊和山羊群以两次为间隔的两次疫情采集的尸检样本中的血琼脂上分离的。通过常规培养技术和聚合酶链反应（PCR）确认了分离物的身份。 PCR后，使用针对cya基因，荚膜基因，S层染色体区域和炭疽芽孢杆菌特异性染色体标记物的特异性引物-Ba813（分别为929、846、639和168 bp），扩增出预期大小的产物。与常规培养技术相比，还强调了这种分子技术在快速和特异性诊断潜在的人畜共患传染病中的重要性。

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