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首页> 外文期刊>Brain research >Transplanted neuronal progenitor cells in a peripheral nerve gap promote nerve repair.
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Transplanted neuronal progenitor cells in a peripheral nerve gap promote nerve repair.

机译：周围神经间隙中移植的神经元祖细胞可促进神经修复。

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A basic experiment of peripheral nerve regeneration using neuronal progenitor cells embedded in collagen gel was performed in a rat sciatic nerve defect. First, when neuronal progenitor cells derived from the fetal rat hippocampus were cultured in atelocollagen-containing medium, neurospheres positive for anti-nestin antibody were confirmed after 8 days. These cells differentiated into astrocytes positive for anti-glial fibrillary acidic protein (GFAP) antibody, oligodendrocytes positive for anti-galactocerebroside (GalC) antibody and neurons positive for anti-neurofilament 200 (NF200) antibody, and they were capable of extending axons. They also differentiated into Schwann-like supportive cells positive for anti-s100 and anti-p75 antibody. Next, a 15-mm defect was prepared in the sciatic nerve of mature rats, and the nerve was bridged with a silicone tube filled with neuronal progenitor cells (1x10(5)) embedded in collagen gel. The transplanted neuronal progenitor cells were labeled in advance with 5-bromo-2-deoxyuridine (BrdU). When the regenerated tissue was examined 6 weeks and 10 weeks after grafting, the number and diameter of myelinated fibers were significantly increased compared with a control tube without neuronal progenitor cells. Action potentials were detected in the regenerated nerve. Also, cells positive for both anti-BrdU antibody and anti-S100 or anti-p75 antibody were observed in the regenerated tissue, and part of the grafted neural stem cells were considered to have differentiated into Schwann cell-like supportive cells. From these results neuronal progenitor cells derived from the fetal rat hippocampus are considered to retain their proliferative and differentiating abilities in collagen gel, and when transplanted to a site of peripheral nerve defect, part of them differentiate into supportive cells and they contributed to promotion of axonal regeneration.
机译：在大鼠坐骨神经缺损中使用胶原蛋白凝胶中嵌入的神经元祖细胞进行周围神经再生的基础实验。首先，当在含端胶原的培养基中培养源自胎儿大鼠海马的神经元祖细胞时，在8天后确认抗Nestin抗体阳性的神经球。这些细胞分化为抗神经胶质纤维酸性蛋白（GFAP）抗体呈阳性的星形胶质细胞，抗半乳糖脑苷脂（GalC）抗体呈阳性的少突胶质细胞和抗神经丝200（NF200）抗体呈阳性的神经元，它们能够扩展轴突。他们还分化为对s100和抗p75抗体呈阳性的雪旺氏样支持细胞。接下来，在成熟大鼠的坐骨神经中准备一个15毫米的缺损，并用填充有嵌入胶原蛋白凝胶中的神经元祖细胞（1x10（5））的硅胶管桥接神经。预先用5-溴-2-脱氧尿苷（BrdU）标记移植的神经元祖细胞。当在移植后6周和10周检查再生组织时，与没有神经元祖细胞的对照管相比，髓鞘纤维的数量和直径显着增加。在再生神经中检测到动作电位。另外，在再生组织中观察到了对抗BrdU抗体和抗S100或抗p75抗体均为阳性的细胞，并且认为部分移植的神经干细胞已经分化为雪旺氏细胞样支持细胞。根据这些结果，认为胎儿大鼠海马神经元祖细胞在胶原蛋白凝胶中保留了它们的增殖和分化能力，当移植到周围神经缺损部位时，它们的一部分分化为支持细胞，并促进了轴突的生长。再生。

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