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首页> 外文期刊>Indian Journal of Small Ruminants >MOLECULAR CHARACTERIZATION OF SHIGA TOXIGENIC Escherichia coli ISOLATES FROM SHEEP
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MOLECULAR CHARACTERIZATION OF SHIGA TOXIGENIC Escherichia coli ISOLATES FROM SHEEP

机译：绵羊志贺毒素大肠埃希菌分离物的分子鉴定

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Shiga toxigenic Escherichia coli (STEC) are commonly found in faeces of food animals and pose threat to human and livestock. The prevalence and characterization of STEC on the basis of virulence genes from lambs with and without diarrhea was studied.A total of 50 E. coli isolates belonging to 13 different serogroups were isolated from 40 diarrhoeic and 31 healthy lambs. All the isolates were screened for the presence of stxh stx2, eae and hlyA genes using multiplex polymerase chain reaction (PCR). Atotal of 15 (30%) (9 diarrhoeic and 6 healthy) E. coli strains were designated as Shiga toxigenic E. coli. Among 15 STEC isoltes, 26.7%, 20%, 46.1% and 80% were found to harbour stxh stx2, eae and hlyA genes respectively. All STEC were further characterized by the production of turbid zones of hemolysis on Soyabean casein digest agar supplemented with 5% sheep erythrocytes and 10 mM CaCl2.
机译：志贺毒原性大肠杆菌（STEC）通常在食用动物的粪便中发现，对人类和牲畜构成威胁。研究了来自有腹泻和没有腹泻的羔羊的毒力基因的STEC的流行情况和特征。从40个腹泻的羔羊和31个健康的羔羊中共分离了50种属于13个不同血清群的大肠杆菌。使用多重聚合酶链反应（PCR）筛选所有分离株中是否存在stxh，stx2，eae和hlyA基因。总共15个（30％）（9个腹泻和6个健康）大肠杆菌菌株被指定为志贺毒素性大肠杆菌。在15种STEC分离物中，发现分别含有stxh stx2，eae和hlyA基因的26.7％，20％，46.1％和80％。所有STEC的特征还在于在补充了5％绵羊红细胞和10 mM CaCl2的大豆酪蛋白消化琼脂上产生混浊的溶血区。

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