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首页> 外文期刊>Brain research >Differential effects of natural polyphenols on neuronal survival in primary cultured central neurons against glutamate- and glucose deprivation-induced neuronal death.
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Differential effects of natural polyphenols on neuronal survival in primary cultured central neurons against glutamate- and glucose deprivation-induced neuronal death.

机译：天然多酚对原代培养的中枢神经元针对谷氨酸和葡萄糖剥夺引起的神经元死亡的神经元存活的差异作用。

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Neuronal injury in the central nervous system following ischemic insult is believed to result from glutamate toxicity and glucose deprivation. In this study, polyphenols isolated from Scutellaria baicalensis Georgi, including baicalin, baicalein, and wogonin, were investigated for their neuroprotective effects against glutamate/NMDA (Glu/NMDA) stimulation and glucose deprivation in primary cultured rat brain neurons. Cell death was accessed by lactate dehydrogenase (LDH) release assay for necrosis, and mitochondrial activity was accessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction activity assay. It was found that both baicalin and baicalein decreased LDH release of the cultured neurons after 24 h treatment, whereas wogonin profoundly increased LDH release after 2 h treatment and resulted in neuronal death after 24 h. Glu/NMDA treatment profoundly increased LDH release and moderately decreased MTT reduction activity in an NMDA receptor-dependent manner. Both baicalin and baicalein significantly reduced Glu/NMDA-increased LDH release, in which baicalein is much more potent than baicalin. Glu/NMDA-increased intracellular calcium was also significantly attenuated by baicalin and baicalein. Baicalin and baicalein did not affect glutamate receptor binding activity, but baicalein did moderately decrease Glu/NMDA-induced nitric oxide (NO) production. In the glucose deprivation (GD) study, baicalein but not baicalin showed significant protective effects on the GD-increased LDH release, without affecting the GD-induced NO production, in cultured rat brain neurons. These results suggest that baicalein is the most effective compound among three polyphenols tested in preventing neurotoxicity induced by both glutamate and GD, whereas baicalin was only effective in preventing glutamate toxicity. Wogonin might have a neurotoxic effect on the brain.
机译：缺血性损伤后中枢神经系统中的神经元损伤被认为是由于谷氨酸毒性和葡萄糖缺乏引起的。在这项研究中，研究了从黄cut中分离出的多酚，包括黄ical苷，黄ical素和沃戈宁，对谷氨酸/ NMDA（Glu / NMDA）刺激和原代培养的大鼠脑神经元葡萄糖剥夺的神经保护作用。细胞死亡通过乳酸脱氢酶（LDH）释放测定坏死，线粒体活性通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑（MTT）还原活性测定。发现黄ical苷和黄ical苷均在24 h处理后降低了培养的神经元的LDH释放，而沃戈宁在2 h处理后显着增加了LDH的释放并导致24 h后神经元死亡。 Glu / NMDA处理以NMDA受体依赖性方式显着增加LDH释放，并适度降低MTT还原活性。黄ical苷和黄ical苷都显着降低了Glu / NMDA增加的LDH释放，其中黄ical苷比黄ical苷更有效。黄ical素和黄ical素也显着减弱了Glu / NMDA增加的细胞内钙。黄ical素和黄ical素不影响谷氨酸受体的结合活性，但黄e素确实适度降低了Glu / NMDA诱导的一氧化氮（NO）的产生。在葡萄糖剥夺（GD）研究中，黄ical苷对培养的大鼠脑神经元中GD增加的LDH释放显示出显着的保护作用，但不影响GD诱导的NO的产生。这些结果表明，黄ical苷是在测试的三种多酚中最有效的化合物，可预防谷氨酸和GD引起的神经毒性，而黄ical苷仅可有效预防谷氨酸的毒性。 Wogonin可能对大脑有神经毒性作用。

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