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首页> 外文期刊>Brain research >Ionic changes accompanying astrocytic intercellular calcium waves triggered by mechanical cell damaging stimulation.
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Ionic changes accompanying astrocytic intercellular calcium waves triggered by mechanical cell damaging stimulation.

机译：机械细胞破坏性刺激触发的伴随星形细胞间质钙波的离子变化。

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摘要

Mechanically poking or damaging a single cell within a confluent astrocyte culture produces the so-called intercellular calcium (Ca(2+)) waves, that is, cell-to-cell propagating changes of intracellular free Ca(2+). We were interested whether intercellular Ca(2+) waves are also associated with changes in other intra- or extracellular ions. To that purpose, we investigated spatiotemporal changes of intracellular Ca(2+) (Ca(i)2+), sodium (Na(i)+) and protons (H(i)+) in primary cultures of rat cortical astrocytes using microfluorescence imaging with fura-2, SBFI and BCECF, respectively; changes of extracellular potassium (K(e)+) were monitored with K(+)-sensitive microelectrodes. Mechanical damage to a single cell by stimulation with a piezo-electrically driven micropipette initiated intercellular Ca(2+) waves that propagated to about 160 microm away from the stimulation point. Na(i)(+) increases could be detected in cells located 2-3 cell diameters from the stimulated cell, acidification was observed 1-2 cell diameters away and Ke(+) increases were measured up to 75 microm away. Kinetic analysis suggests that the Na(i)(+) and H(i)(+) changes occur after, and thus secondary to the Ca(i)(2+) changes. In contrast, K(e)(+) changes occurred very fast, even before the Ca(i)(2+) changes, but their propagation speed was too fast to implicate them as a trigger of Ca(i)(2+) changes. As Na(i)(+) is an important regulator of glycolysis in astrocytes, we hypothesize that astrocytic Na(i)(+) changes in cells located remotely from a damaged cell might be a signal that activates glycolysis thereby producing more lactate that is transferred to the neurons and increases their energy potential to survive the inflicted damage.
机译：机械戳戳或破坏汇合星形胶质细胞培养物中的单个细胞会产生所谓的细胞间钙（Ca（2+））波，即细胞间传播的细胞内游离Ca（2+）变化。我们感兴趣的是细胞间Ca（2+）波是否也与其他细胞内或细胞外离子的变化有关。为此，我们调查了大鼠皮质星形胶质细胞的原代培养物中使用微荧光的细胞内Ca（2+）（Ca（i）2+），钠（Na（i）+）和质子（H（i）+）的时空变化分别用fura-2，SBFI和BCECF成像;用K（+）敏感的微电极监测细胞外钾（K（e）+）的变化。通过压电驱动的微量移液器的刺激对单个细胞的机械损伤引发细胞间Ca（2+）波，传播到距刺激点约160微米。 Na（i）（+）的增加可以在距受刺激细胞2-3个细胞直径处的细胞中检测到，酸化现象在1-2个细胞直径处观察到，而Ke（+）的增加可以测量到75微米。动力学分析表明Na（i）（+）和H（i）（+）的变化发生在Ca（i）（2+）的变化之后，因此是继发的。相反，即使在Ca（i）（2+）发生变化之前，K（e）（+）的变化也发生得非常快，但是它们的传播速度过快，无法暗示它们是Ca（i）（2+）的触发因素变化。由于Na（i）（+）是星形胶质细胞中糖酵解的重要调节剂，我们假设远离受损细胞的细胞中星形细胞Na（i）（+）的变化可能是激活糖酵解的信号，从而产生更多的乳酸。转移到神经元并增加它们的能量潜能，使其免受伤害。

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