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首页> 外文期刊>Indian Journal of Poultry Science >Assessment of generic differences between and within four avian species by employing randomly amplified polymorphic DNA markers.
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Assessment of generic differences between and within four avian species by employing randomly amplified polymorphic DNA markers.

机译：通过使用随机扩增的多态性DNA标记来评估四个禽类之间及其内部的一般差异。

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摘要

Molecular genetic analysis were carried out on 4 important avian species viz. Turkey, Emu, Duck and Chicken (Dahlem Red) as sampled from the genetic stocks maintained at the Department of Poultry Science, ANGRAU and Project Directorate on Poultry, Hyderabad, using Randomly amplified polymorphic DNA (RAPD) markers. Representative samples inclusive of both sexes from each of these species were subjected to RAPD analyses as per standard protocols, using 6 highly informative random primers (decamers) chosen from a total of 24 primers. A cumulative total of 161 polymorphic markers scored from these genetic samples were applied for making intra and inter generic comparison. The results revealed significantly variant and distinct fingerprint patterns specific to each of the species, which aided in instant differentiation of the species and the individuals within the respective species. Within-genera analysis of the samples indicated highest amount of genetic homogeneity for the Dahlem Red (chicken) samples (84.7% genetic similarity), while the ducks were observed to be the least homogeneous as a genetic group (76.7%). Bulk of the RAPD alleles amplified from these primers were representative of the variable regions of the respective genomes, across the genera. The inter-generic distances were very high between the species. When measured, the chicken (Dahlem Red) samples were at a maximum genetic distance from the Turkeys (0.4599) while Ducks were at minimum genetic distance (0.3675) from Emu. The study proved that the RAPD markers were useful as genetic markers for inter-generic variability-assessments, which can form an efficient and affordable marker system for the purposes.
机译：对4种重要禽类进行了分子遗传分析。使用随机扩增的多态性DNA（RAPD）标记，从安格拉市家禽科学部和海得拉巴家禽项目局的基因库中取样的土耳其，E，鸭和鸡（达勒姆红）。按照标准规程，使用选自24种引物中的6种高度信息丰富的随机引物（十聚体），对包括这些物种中每个性别的代表性样品进行RAPD分析。从这些遗传样本中累计获得的总共161个多态性标记被用于进行内部和内部通用比较。结果揭示了每个物种特有的显着变异和独特的指纹图案，这有助于物种和各个物种内的个体的即时分化。样品的属内分析表明，达勒姆红（鸡肉）样品的遗传同质性最高（遗传相似性为84.7％），而鸭的遗传同质性最低（76.7％）。从这些引物扩增得到的大部分RAPD等位基因代表了整个属中各个基因组的可变区。物种之间的属间距离非常高。测量时，鸡（达勒姆红）样本与土耳其的遗传距离最大（0.4599），而鸭子与E的遗传距离最小（0.3675）。该研究证明，RAPD标记可用作跨基因变异性评估的遗传标记，可以为此目的形成高效且负担得起的标记系统。

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