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首页> 外文期刊>Indian Journal of Poultry Science >Incidence of parthenogenetic development in virgin turkey hen's egg
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Incidence of parthenogenetic development in virgin turkey hen's egg

机译：处女火鸡鸡蛋孤雌发育的发生率

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摘要

Present study was conducted to assess the incidence of parthenogenetic development in BSW turkey eggs. The parthenogenetic eggs (from 30wk virgin turkey hens) and normal fertile eggs (by pen mating) were collected daily set in an incubator at a temperature of 37.5°C and 60% relative humidity. The eggs were broken at periodic intervals (0, 12, 24, 36, 48, 60, 72, 84 and 96 h of incubation) and examined macroscopically to record the parthenogenetic development (Cat-1: organised sheath of cells with intact membrane; Cat-2:un-organised sheath of cells with intact membrane; Cat-3: un-organised sheath of cells dissolve membrane and Cat-4: well formed embryo), diameter and total genomic DNA content of blastodisc/embryo). Cat -1 type development was higherduring first 24 h of incubation, whereas, Cat -2 type development was seen from 36 to 84 h of incubation. The Cat -3 and Cat-4 type developments were observed after 60 h of incubation. During first 48 h of incubation Cat-1 and Cat-2 type developments were 19.9 and 3.17% , respectively, while during 48 to 96 h of incubation 8.5, 8.2, 4.6 and 3.25% eggs showed Cat-1, Cat-2, Cat-3 and Cat-4 types of development, respectively. Overall 24% eggs showed posive development. Diameter of blastodisc/ embryo was significantly higher in fertile egg compared to parthenogenetic egg after 12 h of incubation. Genomic DNA content was higher in normal fertilized egg as compared to parthenogenetic egg after 36 h of incubation. It was concluded that embryo development wasslower in parthenogenetic eggs.
机译：进行本研究以评估BSW火鸡卵孤雌生殖发育的发生率。每天在温度为37.5°C和相对湿度60％的恒温箱中收集孤雌卵（来自30wk的原始火鸡母鸡）和正常可育卵（通过笔交配）。卵以周期性间隔（孵化0、12、24、36、48、60、72、84和96 h）破碎，并进行肉眼检查以记录孤雌生殖发育（Cat-1：具有完整膜的组织化细胞鞘; Cat-2：具有完整膜的细胞的无组织鞘; Cat-3：溶解膜的细胞的无组织鞘; Cat-4：胚的/胚的直径和总基因组DNA含量。在培养的最初24小时内，Cat -1类型的发育较高，而在培养的36至84 h内则观察到Cat -2类型的发育。孵育60小时后，观察到Cat -3和Cat-4类型的发育。在孵化的最初48小时内，Cat-1和Cat-2类型的发育分别为19.9和3.17％，而在孵化的48至96小时中，8.5、8.2、4.6和3.25％的卵显示Cat-1，Cat-2，Cat -3和Cat-4类型的开发。总体上，有24％的卵显示出发育旺盛的状态。孵化12小时后，与单性生殖卵相比，可育卵中胚盘/胚的直径明显更高。温育36小时后，正常受精卵的基因组DNA含量高于孤雌卵。结论是单性生殖卵的胚胎发育较慢。

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