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首页> 外文期刊>Brain research >Stimulation of CDP-choline synthesis by uridine or cytidine in PC12 rat pheochromocytoma cells.
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Stimulation of CDP-choline synthesis by uridine or cytidine in PC12 rat pheochromocytoma cells.

机译：尿苷或胞苷在PC12大鼠嗜铬细胞瘤细胞中刺激CDP-胆碱合成。

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摘要

Oral administration of CDP-choline to rats raises plasma and brain cytidine levels and increases brain levels of phosphatidylcholine (PC). In contrast, in humans oral CDP-choline increases plasma levels of uridine. To determine whether uridine can also enhance PC synthesis, we developed an assay for CDP-choline, an immediate and rate-limiting precursor in PC synthesis, and measured this intermediate in clonal PC12 rat pheochromocytoma cells incubated with various concentrations of uridine or cytidine. Addition of uridine (50-100 microM) to the incubation medium caused significant elevations in UTP, CT, USAP and CDP-choline levels in PC12 cells. Uridine had no effect on the synthesis of diacylglycerol (DAG) or the activity of the phosphotransferase which catalyzes the synthesis of PC from DAG and CDP-choline. Hence uridine treatment was unlikely to inhibit the conversion of endogenous CDP-choline to PC. These results suggest the possibility that uridine may also enhance PC synthesis in intact brain.
机译：对大鼠口服CDP胆碱可提高血浆和脑胞苷水平，并增加脑磷脂酰胆碱（PC）的水平。相反，在人类中，口服CDP-胆碱会增加尿苷的血浆水平。为了确定尿苷是否还可以增强PC合成，我们开发了一种CDP-胆碱的测定方法，CDP-胆碱是PC合成中的直接和限速前体，并在与各种浓度的尿苷或胞苷孵育的PC12大鼠嗜铬细胞瘤细胞中测定了该中间体。向培养液中添加尿苷（50-100 microM）导致PC12细胞中UTP，CT，USAP和CDP-胆碱水平显着升高。尿苷对二酰基甘油（DAG）的合成或催化由DAG和CDP-胆碱合成PC的磷酸转移酶的活性没有影响。因此，尿苷处理不太可能抑制内源性CDP-胆碱向PC的转化。这些结果表明，尿苷还可能增强完整大脑中PC的合成。

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