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首页> 外文期刊>Brain research >Microglial responses in the avascular quail retina following transection of the optic nerve.
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Microglial responses in the avascular quail retina following transection of the optic nerve.

机译：视神经横断后，无血管鹌鹑视网膜中的小胶质细胞反应。

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This study was undertaken to investigate microglial responses in the avascular central nervous system using the quail retina that is known to be devoid of blood vessels. Following intraorbital optic nerve transection (ONT), the quail retina was examined immunohistochemically at various times up to 6 months. A few days after transection, microglia in the inner retinal layers revealed features of activation. Activated cells displayed an amoeboid shape and enhanced QH1-immunoreactivity. The numbers of these amoeboid cells were rapidly increased, first in the inner plexiform layer (IPL), and then in the ganglion cellerve fiber layer (GCL/NFL) of the retina where retrograde degenerating ganglion cell processes and perikarya were located. By 6 months after transection, microglia regained their resting morphology, and their cell counts returned to control levels. At early time points of microglial activation, numerous QH1+ amoeboid cells were observed along the vitreal surface of the pecten and retinal region adjacent to the insertion of the pecten, where some amoeboid cells were attached underneath the internal limiting membrane, and appeared to squeeze through the optic nerve fiber bundles. A considerable number of these amoeboid cells in the GCL/NFL and the IPL were labeled with PCNA, suggesting that active exogenous migration (from the pecten) and in situ proliferation of precursor cells contribute to the increase in microglial population of the degenerating retina. On the other hand, TUNEL-positive microglia appeared in the GCL/NFL at later time points indicate that the decrease of microglial numbers is in part due to apoptosis in these layers. Although some aspects of microglial activation in the avascular retina appear unique, their consequences were similar to those described in vascular retinae of mammals, a finding indicates that blood vessels are not a prerequisite for microglial activation, and microglial precursors could migrate long distance to reach the lesioned site, which is not accessible via blood vessels. Our data provide the first analysis of microglial activation in the avascular central nervous system (CNS), and suggest that the quail retina is a useful model for studies of microglial behavior in CNS.
机译：这项研究的目的是使用已知没有血管的鹌鹑视网膜来研究无血管中枢神经系统中的小胶质细胞反应。眶内视神经横断（ONT）后，在长达6个月的不同时间对鹌鹑视网膜进行免疫组织化学检查。横切几天后，视网膜内层的小胶质细胞显示出激活的特征。活化的细胞显示出变形虫形状和增强的QH1免疫反应性。这些变形虫细胞的数量迅速增加，首先在内部的丛状层（IPL）中，然后在视网膜的神经节细胞/神经纤维层（GCL / NFL）中，逆行的退化神经节细胞过程和周围核位于其中。横切后6个月，小胶质细胞恢复了静息形态，其细胞计数恢复到对照水平。在小胶质细胞活化的早期，沿果胶的玻璃体表面和邻近果胶插入的视网膜区域观察到大量QH1 +变形虫细胞，其中一些变形虫细胞附着在内部限制膜下，并似乎通过视神经纤维束。 GCL / NFL和IPL中的大量此类变形生物细胞都用PCNA进行了标记，这表明主动的外源迁移（来自果胶）和前体细胞的原位增殖有助于变性视网膜的小胶质细胞数量增加。另一方面，在较晚的时间点，GCL / NFL中出现了TUNEL阳性小胶质细胞，表明小胶质细胞数量的减少部分是由于这些层中的细胞凋亡。尽管无血管视网膜中小胶质细胞活化的某些方面看起来很独特，但其后果与哺乳动物的血管视网膜中描述的结果相似，但发现表明血管不是小胶质细胞活化的先决条件，并且小胶质细胞前体可以迁移很长的距离才能到达小血管。病变部位，无法通过血管进入。我们的数据提供了对无血管中枢神经系统（CNS）中小胶质细胞活化的首次分析，并表明鹌鹑视网膜是研究CNS中小胶质细胞行为的有用模型。

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