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Inductive response of protective enzymes in coconut (Cocos nucifera L.)subjected to stress

机译:胁迫下椰子(Cocos nucifera L.)中保护酶的诱导响应

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摘要

The coconut hybrids WCT x four exotic Tails viz., Philippines Ordinary PHOT, Fiji PUT, Cochin China CCNT, Strait Settlement Green SSGT as well as one indigenous tall- Kappadam KPDT were screened for tolerance to drought by using stomatal resistance and transpiration rate in the field as well as by the activities of the stress sensitive enzymes viz., malic dehydrogenase, peroxidase, super oxide dismutase and catalase under induced stress condition. Leaf water potential Y leaf was determined both in situ and induced stress conditions. Differences in the parameters studied did not give any significant variations among the hybrid combinations during non-stress or stress periods and significant variations were observed only between the two periods under in situ conditions. However, under induced stress condition, the hybrids behaved differentially in activities of the enzymes in response to the decreased F leaf. Based on the increase or decrease in the water relation components as well as stress related enzyme activities as compared with the normal level the hybrids were ranked for tolerance to drought. Ranking of the hybrids based on y leaf under induced stress condition as well as under field stress conditions gave similar results. Amongthe T x T combinations, WCT x SSGT was found to have relative tolerance to drought in terms of water relation components and enzyme activities. The results further established that rapid screening techniques can be employed with ease for screening largenumber of coconut cultivars / hybrids for tolerance to drought.
机译:椰子杂种WCT x四个异国尾巴,即菲律宾普通PHOT,斐济PUT,科钦中国CCNT,海峡殖民地绿色SSGT以及一个土生土长的Kappadam KPDT,通过使用气孔阻力和蒸腾速率来筛选耐旱性。以及在胁迫条件下胁迫敏感酶的活性,即苹果脱氢酶,过氧化物酶,超氧化物歧化酶和过氧化氢酶的活性。在原位和诱导胁迫条件下测定叶水势Y叶。在非胁迫或胁迫时期,所研究参数的差异在杂交组合之间没有任何显着变化,并且仅在原位条件下在两个时期之间观察到显着变化。然而,在诱导的胁迫条件下,杂种响应F叶减少而表现出不同的酶活性。与正常水平相比,基于水分相关成分的增加或减少以及与胁迫相关的酶活性,对杂交品种的耐旱性进行了排名。在诱导胁迫条件下以及在田间胁迫条件下,基于y叶对杂种的排名给出了相似的结果。在T x T组合中,发现WCT x SSGT在水分关系成分和酶活性方面具有相对的耐旱性。结果进一步证实,可以快速采用快速筛选技术来筛选大量椰子品种/杂种以耐受干旱。

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