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首页> 外文期刊>Indian Journal of Horticulture >Effect of in vivo and in vitro seed germination and performance of Lisianthus seedlings.
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Effect of in vivo and in vitro seed germination and performance of Lisianthus seedlings.

机译:体内和体外发芽种子发芽的效果以及莲子的性能。

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摘要

An experiment was conducted under controlled conditions to standardize the temperature, substrates and seed quality on germination in lisianthus cv. Blue Magic using both in vivo and in vitro propagation. In vivo seed germination was conducted at three temperature levels (20+or-1 degrees C; 25+or-1 degrees C and ambient room temp.) and eight substrate media {cocopeat, sand, perlite, blotting sheet, germination paper, cocopeat+perlite (1:1); (2:1) and (1:2)}. For in vitro germination, seeds were inoculated on the MS medium supplemented with 0.5 mg/l IBA and BAP and cultured at controlled conditions (25+or-1 degrees C with 16/8 h light/dark photo period). In one-year-old seeds, maximum seed germination (38.66%) were observed in cocopeat: perlite (1:2) followed by cocopeat: perlite (1:1) at room temperature (34.0%). Under in vitro conditions, maximum seed germination (63.89%) was observed on MS medium supplemented with 0.5 mg/l IBA for fresh seeds.
机译:在受控条件下进行了一项实验,以标准化桔梗发芽的温度,基质和种子质量。同时使用体内和体外传播的Blue Magic。在三个温度水平(20 +或-1摄氏度; 25 +或-1摄氏度和环境室温)和八种基质介质(椰油,沙子,珍珠岩,吸水纸,发芽纸,cocopeat +珍珠岩(1:1); (2:1)和(1:2)}。对于体外发芽,将种子接种在补充有0.5 mg / l IBA和BAP的MS培养基上,并在受控条件下(25 +或-1摄氏度,用16/8 h光照/黑暗照片进行培养)期)。在一年大的种子中,室温下(34.0%)的共钴粉:珍珠岩(1:2),然后是共钴粉:珍珠岩(1:1),观察到最大的种子发芽(38.66%)。在体外条件下,对于新鲜种子,在添加0.5 mg / l IBA的MS培养基上观察到最大的种子发芽率(63.89%)。

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