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首页> 外文期刊>Brain research bulletin >Rhodamine backfilling and confocal microscopy as a tool for the unambiguous identification of neuronal cell types: a study of the neurones of the rat cochlear nucleus.
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Rhodamine backfilling and confocal microscopy as a tool for the unambiguous identification of neuronal cell types: a study of the neurones of the rat cochlear nucleus.

机译：罗丹明回填和共聚焦显微镜作为明确鉴定神经元细胞类型的工具：对大鼠耳蜗核神经元的研究。

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摘要

Adequate interpretation of the functional data characterising the projection neurones of the cochlear nucleus (CN) is impossible without the unequivocal classification of these cell types at the end of the experiments. In this study, morphological criteria applicable for unambiguous identification of CN neurones have been sought. The neurones were labelled with rhodamine from incisions severing the projection pathways of the individual cell types, allowing their selective labelling and morphological characterisation. Confocal microscopy was employed for the investigation of the rhodamine-filled cells whose morphology was assessed after reconstructing the three-dimensional images of the cell bodies and proximal processes. The diameters of the somata and the number of processes originating from the cell bodies were also determined. In most of the cases, unambiguous identification of the bushy, octopus and Purkinje-like cells was relatively straightforward. On the other hand, precise classification of thepyramidal cells was often difficult, especially because giant cells could easily possess morphological features resembling pyramidal neurones. Occasionally, giant cells also mimicked the appearance of octopus neurones, which may be another important source of identification error, especially as these two cell types are often situated close to each other in the CN. It is concluded that morphological criteria defined in the present work may be effectively applied for the unambiguous identification of the projection neurones of the CN, even following functional measurements, when the correct cell classification is essential for the interpretation of the experimental data. Moreover, the present study also confirmed that Purkinje-like cells project to the cerebellum.
机译：如果没有在实验结束时对这些细胞类型进行明确分类，就不可能充分解释表征耳蜗核（CN）投射神经元的功能数据。在这项研究中，寻求适用于CN神经元的明确鉴定的形态学标准。用从切口切开的若丹明标记神经元，切断单个细胞类型的投射途径，从而对其进行选择性标记和形态学表征。共聚焦显微镜用于研究若丹明填充的细胞，在重建细胞体和近端过程的三维图像后评估其形态。还确定了体细胞的直径和源自细胞体的过程数。在大多数情况下，对丛生，章鱼和类浦肯野细胞的明确鉴定是相对简单的。另一方面，通常难以对锥体细胞进行精确分类，特别是因为巨细胞很容易具有类似于锥体神经元的形态特征。有时，巨型细胞也模仿章鱼神经元的出现，这可能是识别错误的另一个重要来源，特别是因为这两种细胞类型通常在CN中彼此靠近。结论是，当正确的细胞分类对于实验数据的解释是必不可少的时，即使在进行功能测量之后，本工作中定义的形态学标准也可以有效地应用于CN投射神经元的明确鉴定。此外，本研究还证实了类浦肯野细胞向小脑突出。

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